FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1183451181> ?p ?o ?g. }

• W1183451181 endingPage "215" @default.
• W1183451181 startingPage "204" @default.
• W1183451181 abstract "While attempting to isolate the enzyme geranylgeraniol 18-hydroxylase, which is involved in plaunotol biosynthesis in Croton stellatopilosus (Cs), the cDNAs for a cytochrome P450 monooxygenase(designated as CYP76F45) and an NADPH-cytochrome P450 reductase (designated as CPR I based on its classification) were isolated from the leaf. The CYP76F45 and CsCPR I genes have open reading frames (ORFs) encoding 507- and 711-amino acid proteins with predicted relative molecular weights of 56.7 and 79.0 kDa,respectively. Amino acid sequence comparison showed that both CYP76F45 (63–73%) and CsCPR I (79–83%) share relatively high sequence identities with homologous proteins in other plant species.Phylogenetic tree analysis confirmed that CYP76F45 belongs to the CYP76 family and that CsCPR I belongs to Class I of dicotyledonous CPRs, with both being closely related to Ricinus communis genes. Functional characterization of both enzymes, each expressed separately in Escherichia coli as recombinant proteins,showed that only simultaneous incubation of the membrane bound proteins with the substrate geraniol (GOH) and the coenzyme NADPH could form 8-hydroxygeraniol. The enzyme mixture could also utilize acyclic sesquiterpene farnesol (FOH) with a comparable substrate preference ratio (GOH:FOH) of 54:46. The levelsof the CYP76F45 and CsCPR I transcripts in the shoots, leaves and twigs of C. stellatopilosus were correlated with the levels of a major monoterpenoid indole alkaloid, identified tentatively as 19-Evallesamine,that accumulated in these plant parts. These results suggested that CYP76F45 and CPR I function as the enzyme geraniol-8-hydroxylase (G8H), which is likely to be involved in the biosynthesis of the indole alkaloid in C. stellatopilosus [corrected]." @default.
• W1183451181 created "2016-06-24" @default.
• W1183451181 creator A5007241763 @default.
• W1183451181 creator A5017626485 @default.
• W1183451181 creator A5029754554 @default.
• W1183451181 creator A5051243841 @default.
• W1183451181 creator A5063558377 @default.
• W1183451181 date "2015-10-01" @default.
• W1183451181 modified "2023-10-14" @default.
• W1183451181 title "Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba" @default.
• W1183451181 cites W1520292993 @default.
• W1183451181 cites W1963970760 @default.
• W1183451181 cites W1967520374 @default.
• W1183451181 cites W1969176286 @default.
• W1183451181 cites W1971005452 @default.
• W1183451181 cites W1975214920 @default.
• W1183451181 cites W1985504443 @default.
• W1183451181 cites W1987171157 @default.
• W1183451181 cites W1989060614 @default.
• W1183451181 cites W2003781387 @default.
• W1183451181 cites W2019516171 @default.
• W1183451181 cites W2021608380 @default.
• W1183451181 cites W2021842042 @default.
• W1183451181 cites W2030910942 @default.
• W1183451181 cites W2047180184 @default.
• W1183451181 cites W2055309018 @default.
• W1183451181 cites W2056953793 @default.
• W1183451181 cites W2070099755 @default.
• W1183451181 cites W2076991203 @default.
• W1183451181 cites W2077929706 @default.
• W1183451181 cites W2102005894 @default.
• W1183451181 cites W2108922833 @default.
• W1183451181 cites W2127294029 @default.
• W1183451181 cites W2140298738 @default.
• W1183451181 cites W2152207030 @default.
• W1183451181 cites W4240181774 @default.
• W1183451181 cites W4254275792 @default.
• W1183451181 doi "https://doi.org/10.1016/j.phytochem.2015.08.005" @default.
• W1183451181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26300313" @default.
• W1183451181 hasPublicationYear "2015" @default.
• W1183451181 type Work @default.
• W1183451181 sameAs 1183451181 @default.
• W1183451181 citedByCount "7" @default.
• W1183451181 countsByYear W11834511812017 @default.
• W1183451181 countsByYear W11834511812020 @default.
• W1183451181 countsByYear W11834511812021 @default.
• W1183451181 countsByYear W11834511812022 @default.
• W1183451181 countsByYear W11834511812023 @default.
• W1183451181 crossrefType "journal-article" @default.
• W1183451181 hasAuthorship W1183451181A5007241763 @default.
• W1183451181 hasAuthorship W1183451181A5017626485 @default.
• W1183451181 hasAuthorship W1183451181A5029754554 @default.
• W1183451181 hasAuthorship W1183451181A5051243841 @default.
• W1183451181 hasAuthorship W1183451181A5063558377 @default.
• W1183451181 hasConcept C104317684 @default.
• W1183451181 hasConcept C13002179 @default.
• W1183451181 hasConcept C134651460 @default.
• W1183451181 hasConcept C148292235 @default.
• W1183451181 hasConcept C171305022 @default.
• W1183451181 hasConcept C181199279 @default.
• W1183451181 hasConcept C193252679 @default.
• W1183451181 hasConcept C2777278459 @default.
• W1183451181 hasConcept C2778240251 @default.
• W1183451181 hasConcept C2780768313 @default.
• W1183451181 hasConcept C526171541 @default.
• W1183451181 hasConcept C547475151 @default.
• W1183451181 hasConcept C55493867 @default.
• W1183451181 hasConcept C59822182 @default.
• W1183451181 hasConcept C86803240 @default.
• W1183451181 hasConceptScore W1183451181C104317684 @default.
• W1183451181 hasConceptScore W1183451181C13002179 @default.
• W1183451181 hasConceptScore W1183451181C134651460 @default.
• W1183451181 hasConceptScore W1183451181C148292235 @default.
• W1183451181 hasConceptScore W1183451181C171305022 @default.
• W1183451181 hasConceptScore W1183451181C181199279 @default.
• W1183451181 hasConceptScore W1183451181C193252679 @default.
• W1183451181 hasConceptScore W1183451181C2777278459 @default.
• W1183451181 hasConceptScore W1183451181C2778240251 @default.
• W1183451181 hasConceptScore W1183451181C2780768313 @default.
• W1183451181 hasConceptScore W1183451181C526171541 @default.
• W1183451181 hasConceptScore W1183451181C547475151 @default.
• W1183451181 hasConceptScore W1183451181C55493867 @default.
• W1183451181 hasConceptScore W1183451181C59822182 @default.
• W1183451181 hasConceptScore W1183451181C86803240 @default.
• W1183451181 hasLocation W11834511811 @default.
• W1183451181 hasLocation W11834511812 @default.
• W1183451181 hasOpenAccess W1183451181 @default.
• W1183451181 hasPrimaryLocation W11834511811 @default.
• W1183451181 hasRelatedWork W11379959 @default.
• W1183451181 hasRelatedWork W1586135266 @default.
• W1183451181 hasRelatedWork W2046376689 @default.
• W1183451181 hasRelatedWork W2053788548 @default.
• W1183451181 hasRelatedWork W2067042487 @default.
• W1183451181 hasRelatedWork W2098492595 @default.
• W1183451181 hasRelatedWork W2228086802 @default.
• W1183451181 hasRelatedWork W2412452973 @default.
• W1183451181 hasRelatedWork W58184174 @default.
• W1183451181 hasRelatedWork W967886486 @default.

• next