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- W118563509 abstract "We investigated the biosynthesis of mixed-linkage {beta}-D-glucan and glucuronoarabinoxylans which make up the hemicellulosic matrix of the primary cell walls of maize and other cereal grasses. The Golgi apparatus was enriched from plasma membrane and other organelles by flotation density gradient centrifugation. Glucan synthase I and II, which are established markers for Golgi and plasma membrane, respectively, displayed considerable overlap in conventional separations with sucrose density gradients. Flotation gradients improved separation of the membranes substantially, but the different synthases themselves also incorporated radioactivity from either 10 {mu}M or 1 mM UDP-({sup 14}C)-glucose into polymer. Relative incorporation of radioactivity into polymers from UDP-({sup 14}C)-xylose by the various membrane fractions was nearly identical to relative IDPase activities, indicating that combined xylosyl transferase-xylan synthase represents a new, unequivocal marker for the Golgi apparatus. We also have developed techniques of gas-liquid chromatography and radiogas proportional counting to achieve capillary quality separation of partially methylated alditol acetates with simultaneous determination of radioactivity in the derivatives. Digestion of polymeric products by specific endo-glycanohydrolases to diagnostic oligosaccharides also reveal specific kinds of polysaccharides synthesized by the Golgi membranes. A combination of these techniques provides unequivocal determination of the linkage structure of specific polymers synthesized by the purifiedmore » Golgi apparatus.« less" @default.
- W118563509 created "2016-06-24" @default.
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- W118563509 date "1989-04-01" @default.
- W118563509 modified "2023-09-27" @default.
- W118563509 title "Synthesis of cell wall xylans and glucans by golgi membranes" @default.
- W118563509 hasPublicationYear "1989" @default.
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