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- W1192402868 abstract "This chapter describes the techniques for growing murine leukemia viruses in tissue culture and discusses methods for purifying virus in sufficient quantities for subsequent biochemical studies. As the success of these techniques involves determinations of viral titer, several of the commonly used, quantitative in vitro assays for viral replication are also described. Murine type C viruses have been classified into two major groups based on their potential for producing neoplastic disease. The mouse leukemia viruses (MuLVs) can replicate in cultures of fibroblastic and epithelioid cells and generally do not produce cytopathic effects or morphological transformation. Mouse sarcoma viruses (MSVs) are defective in their ability to replicate, require “helper” MuLVs for their continued propagation in cell culture, and produce morphological transformation of susceptible cells in vitro." @default.
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- W1192402868 date "1979-01-01" @default.
- W1192402868 modified "2023-09-25" @default.
- W1192402868 title "[35] Purification and assay of murine leukemia viruses" @default.
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- W1192402868 doi "https://doi.org/10.1016/s0076-6879(79)58156-7" @default.
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