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- W11962089 abstract "Publisher Summary 3-Hydroxyanthranilic acid (3-OH-A) is an intermediate in the catabolism of tryptophan and is excreted in urine. This substance, which is carcinogenic in animal experiments, may be involved in the pathogenesis of bladder cancer in humans. Until now, the determination of 3-OH-A has required its isolation by extraction of the urine and separation by chromatographic methods followed by colorimetric or fluorimetric assay with variable recoveries. This chapter describes an enzymatic method that is used for the determination of 3-OH-A. This method is applicable in biochemistry and clinical chemistry. In this method, 3-OH-A is oxidized by 3-hydroxyanthranilic acid oxidase to 2-amino-3-carboxymuconate semialdehyde. 3-OH-A can be determined by its absorption maximum at 360 nm in that method. The chapter reviews the entire procedure of this method, including the principle, reagents, solutions, and optimum conditions used in it. It also reviews the preparation of the solutions used in the method and the stability of these solutions. The method has its own accuracy, precision, and specificity. However, there are some sources of error in the method as well." @default.
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- W11962089 date "1974-01-01" @default.
- W11962089 modified "2023-09-27" @default.
- W11962089 title "3-Hydroxyanthranilic Acid" @default.
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- W11962089 doi "https://doi.org/10.1016/b978-0-12-091304-6.50022-7" @default.
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