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- W1197777386 abstract "Publisher Summary In vitro perifusion of hypothalamic or pituitary tissue for the purpose of investigating mechanisms of hypothalamohypophyseal neuroendocrine regulation is a methodology that complements in vivo as well as alternative in vitro techniques, and it circumvents many of their disadvantages. For example, although methods for the collection of hypothalamohypophyseal portal blood have been developed that allow direct quantification of regulatory factor (neurotransmitter, neuromodulator, and releasing factor) release into the portal flow, these techniques usually require use of systemic anesthesia (which must be assumed to alter normal interneuronal dynamics within the hypothalamus) and permit only the activity of a relatively small population of neurons that secrete into the portal flow to be monitored. In vitro perifusion of human hypothalamic and pituitary tissue provides a valuable model for the investigation of hypothalamohypophyseal regulation and interactions that cannot otherwise be directly evaluated in humans. As with other in vitro techniques, this methodology provides a complementary approach to in vivo investigations in both humans and laboratory animals." @default.
- W1197777386 created "2016-06-24" @default.
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- W1197777386 date "1989-01-01" @default.
- W1197777386 modified "2023-09-26" @default.
- W1197777386 title "In vitro perifusion of human hypothalamic and pituitary tissue" @default.
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- W1197777386 doi "https://doi.org/10.1016/0076-6879(89)68014-7" @default.
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