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- W1199898208 abstract "This chapter describes the purification of calmodulin from plant tissues. Plant tissues in general have low protein-to-fresh weight ratios and can be high in proteases, quinones, phenolics, pigments, and other secondary products. All these contaminants can lead to both poor yields and poor-quality calmodulin. The chapter discusses a procedure to produce large quantities of pure plant calmodulin with minimum effort. The key to purifying most calmodulins, including plant calmodulin, has been the development of phenothiazine-Sepharose affinity resins. The procedure for the isolation of plant calmodulin involves dry seeds as the starting material. However, the procedure has proved to be suited to a large number of starting tissues, including animal brain tissue. Modifications required for different tissues are noted in the procedure. For small quantities of wheat germ calmodulin, the procedure can be abbreviated to homogenization, batch adsorption on diethylaminoethyl (DEAE)-cellulose, and affinity chromatography. Using an acetone extraction has the advantage of removing lipid material from the tissue. When the tissue is homogenized directly without prior acetone extraction, greater precautions should be taken to control phenolic oxidation. Sodium metabisulfite at concentrations up to 0.4 mM may be added to the homogenization buffer in addition to 2-mecaptoethanol to prevent oxidation." @default.
- W1199898208 created "2016-06-24" @default.
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- W1199898208 date "1983-01-01" @default.
- W1199898208 modified "2023-10-18" @default.
- W1199898208 title "[2] Purification of plant calmodulin" @default.
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- W1199898208 doi "https://doi.org/10.1016/s0076-6879(83)02004-2" @default.
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- W1199898208 hasPublicationYear "1983" @default.
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