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- W1200675848 abstract "The PKC1 cell wall integrity pathway (CWIP) is essential for survival and maintenance of cell wall integrity in yeast cells. Upon exposure to cell wall stress, signaling proteins (sensors) in the plasma membrane activate this pathway by interacting with other signaling proteins (interactors). The objective of this research is to know if interactors previously identified by the integrated Membrane Yeast Two-Hybrid (iMYTH) technique contribute to CWIP activation under thermal stress. Our hypothesis is that interactors of the Wsc1p and Mid2p stress sensors are required for survival to thermal stress. To test this hypothesis, strains bearing null mutations for genes encoding stress sensor proteins and their interacting protein partners were tested for CWIP activation at 37°C by Western Blot technique. The cells were exposed 1hr at 37°C during log phase and the protein extracts were tested with antibodies that measure CWIP activation through Slt2p phosphorylation. Most of the null mutants showed normal PKC1 activation when shifted from growth at 27°C to 37°C similar to a wild type strain. Deletion of GTT1 an interactor of Mid2p exhibited very high levels of Slt2p phosphorylation in both conditions relative to the wild type suggesting that PKC1 was being activated constitutively. In conclusion, interactors of Wsc1p and Mid2p can function as signaling proteins involved in PKC1 pathway regulation and may be associated with survival under different stresses. This research was supported by the UPR-MSC, Univ. of Toronto, Univ. of Kentucky, NIGMS/NIAID-SC1AI081658, NCRR-RCMI-G12MD007600, MBRS-RISE-R25GM061838, and NIH-INBRE 8 P20 GM103475-13." @default.
- W1200675848 created "2016-06-24" @default.
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- W1200675848 date "2015-04-01" @default.
- W1200675848 modified "2023-10-01" @default.
- W1200675848 title "Biochemical Analysis of Wsc1p and Mid2p Interacting Partners under Thermal Stress" @default.
- W1200675848 doi "https://doi.org/10.1096/fasebj.29.1_supplement.728.43" @default.
- W1200675848 hasPublicationYear "2015" @default.
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