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- W120108711 abstract "Microarrays aim at measuring the expression degree of thousands of genes in a single experiment. The measured intensities of the probe spots are unfortunately affected by parasitic interferences, which in many cases prohibit the direct translation into the target RNA concentrations. The calibration of raw intensity data is therefore a fundamental prerequisite for the analysis of oligonucleotide microarray data. The field of array normalization tools can be separated into tools that use only the perfect match probes (PMs) of one chip, tools that use PMs and mismatch probes (MMs) of one chip and tools that rely on multichip normalization techniques and reference arrays. The best results are yielded by multichip techniques like RMA or GCRMA. However, it is often unwanted to hybridize multiple arrays. We report a method of data adjustment based on a physico-chemical model which corrects raw microarray intensity data of one chip for the effect of (i) sequence-specific affinities, (ii) mismatches, (iii) cross-hybridization and (iv) saturation [3, 1, 2]." @default.
- W120108711 created "2016-06-24" @default.
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- W120108711 date "2006-01-01" @default.
- W120108711 modified "2023-09-27" @default.
- W120108711 title "A new program package for model-based microarray analysis" @default.
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