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- W1201472359 abstract "This chapter describes the determination of inositol 1-phosphate synthetase and inositol 1-phosphatase from yeast. The most convenient method of assaying inositol-l-P synthetase is based on the determination of the radioactive inositol 1-P formed enzymatically from 14C-labeled glucose-6-P. The radioactive inositol-l-P present in the incubation mixture is quantitatively converted to free inositol by the action of purified alkaline phosphatase, and the radioactive inositol is isolated by paper chromatography and counted. The assay method for inositol 1-phosphatase method is based on the colorimetrie determination of Pi released enzymatically from L-myo-inositol-l-P. The purified enzyme preparation retains all its synthetase and phosphatase activities for at least 40 days if kept frozen at –20° in the presence of 0.1 mM GSH. Repeated thawing and freezing of the enzyme solution causes progressive inactivation of the synthetase, but not of the phosphatase. The rate of heat inactivation of the synthetase is much faster than that of the phosphatase. Heating at 60° for 8 and 15 minutes destroys 52 and 90% of the synthetase, respectively; under the same conditions the phosphatase is inactivated by 11 and 25%, respectively." @default.
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- W1201472359 date "1966-01-01" @default.
- W1201472359 modified "2023-09-23" @default.
- W1201472359 title "[125] Inositol 1-phosphate synthetase and inositol 1-phosphatase from yeast" @default.
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- W1201472359 doi "https://doi.org/10.1016/0076-6879(66)09141-9" @default.
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