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• W1208253679 startingPage "46" @default.
• W1208253679 abstract "Crystalline silica inhaled from occupational sources has been classified by IARC as carcinogenic to humans; in contrast, for amorphous silica, epidemiological and experimental evidence remains insufficient. The genotoxicity of crystalline silica is still debated because of the inconsistency of experimental results (“variability of silica hazard”), often related to the features of the particle surfaces. We have assessed the role of crystal habit in the genotoxicity of silica powders. Pure quartz (crystalline) and vitreous silica (amorphous), sharing the same surface features, were used in an in vitro study with human pulmonary epithelial (A549) and murine macrophage (RAW264.7) cell lines, representative of occupational and environmental exposures. Genotoxicity was evaluated by the comet and micronucleus assays, and cytotoxicity by the trypan blue method. Cells were treated with silica powders for 4 and 24 h. Quartz but not vitreous silica caused cell death and DNA damage in RAW264.7 cells. A549 cells were relatively resistant to both powders. Our results support the view that crystal habit per se plays a pivotal role in modulating the biological responses to silica particles." @default.
• W1208253679 created "2016-06-24" @default.
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• W1208253679 date "2015-10-01" @default.
• W1208253679 modified "2023-09-28" @default.
• W1208253679 title "Does the crystal habit modulate the genotoxic potential of silica particles? A cytogenetic evaluation in human and murine cell lines" @default.
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• W1208253679 doi "https://doi.org/10.1016/j.mrgentox.2015.07.005" @default.
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• W1208253679 hasPublicationYear "2015" @default.
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