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- W120832596 abstract "Publisher Summary This chapter discusses the use of protein A rosettes to detect cell surface antigen. This method involves treating a cell population with antiserum directed to the surface antigen of interest and then labeling the cells that have bound the antiserum with sheep red cells (SRC). The antigen-bearing cells are, thus, easily visualized in the microscope as rosettes or lymphoid cells surrounded by red cells. Cells in the population that lack the antigen do not bind the antiserum and, therefore, do not bind the indicator SRC. The indicator red cells are produced by coating SRC with protein A (SpA), a 42,000 MW protein that forms part of the cell wall of the bacterium Staphylococcus aureus. This protein binds specifically to the Fc portion of immunoglobulins of the IgG class of several species. Murine peripheral blood lymphocytes are incubated with the appropriate anti-H-2 sera and SpA-SRC. This is a rapid assay method that has the advantage that scoring of the test need be only qualitative: Positive animals will have 80–90% rosettes, whereas negative animals will have less than 5% rosettes, a difference that is obvious from a brief scan of a wet mount." @default.
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- W120832596 date "1979-01-01" @default.
- W120832596 modified "2023-09-27" @default.
- W120832596 title "The Use of Protein A Rosettes to Detect Cell-Surface Antigens" @default.
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