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- W120861906 abstract "Abstract Bronchial epithelial cells are the natural human host for influenza infection but knowledge about the innate immune response is limited. Study objectives; characterise the innate immune response of bronchial epithelial cells to infection with human influenza A virus and relate this to virus replication. Methods; Influenza H3N2, A/Wellington/2004 (H3N2) was cultured and titred using plaque assays on Manine Darby Kidney Cells. Primary bronchial epithelial cells (PBECs) were obtained by bronchoscopy and cultured. Their response was compared to an epithelial cell line, Calu 3. Innate immune response was measured by the induction of mRNA by real time PCR for RIG-I, MDA-5, TLR-3, PKR, interferon (IFN)-β. Protein expression was then measured by western blot in cell lysate or supernatant. Results; H3N2 replicated to significantly higher titres in Calu 3 cells 2.8x106 PFU/ml compared to PBECs 789 PFU/ml (p<0.001). Following infection there was greater than 20 fold induction of RIG-I mRNA in both cells, strong protein expression in PBECs by 24hr, only faint expression in Calu3. A thousand fold induction in MDA-5 mRNA was seen in PBECs at 48hr less than 20 fold in Calu3. Induction of IFN-β mRNA by 24hr and protein release in the supernatant was seen in both cells by 6hr. Conclusions; H3N2 replicates less well in PBECs and this is associated with more vigorous induction of RIG-I during early infection and later induction of MDA-5 mRNA." @default.
- W120861906 created "2016-06-24" @default.
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- W120861906 date "2009-04-01" @default.
- W120861906 modified "2023-09-24" @default.
- W120861906 title "Innate immune response of bronchial epithelial cells to infection with influenza (130.7)" @default.
- W120861906 doi "https://doi.org/10.4049/jimmunol.182.supp.130.7" @default.
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