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- W12088822 abstract "AIM: In order to investigate the pacing mechanism of the interstitial cells of Cajal (ICC), the method for isolation and culture of ICC from the Wistar rat stomach was explored. METHODS: The gastric tissue of Wistar rat was dissected. Collagenase was used to isolate ICC. The cells were sus- pended in smooth muscle cell medium containing stem cell factor. The medium was changed every other day un- til the cells were used. The c-Kit antibody was applied to label ICC to distinguish them from smooth muscle cells. RESULTS: ICC in culture maintained the intrinsic characteristics: multiple processes, large nuclei, and in- tercellular network. Fluorescent staining with c-Kit anti- body confirmed that the culture ICC was successful." @default.
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- W12088822 date "2005-01-01" @default.
- W12088822 modified "2023-09-23" @default.
- W12088822 title "Isolation and Culture of Rat Gastric Interstitial Cells of Cajal" @default.
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- W12088822 doi "https://doi.org/10.11569/wcjd.v13.i4.495" @default.
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