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- W12129628 abstract "Synopsis In order to accumulate inhibitory substance(s) CHO-cells were cultivated in a continuous medium-recycling-process [3] in which 65% of the cultivation medium was re-used. A hybridoma cell line was cultivated in a Super Spinner [1] in repeated batch mode with 100 % medium recycling. Cell death or lower growth rates are indicators for an accumulation of the inhibitor(s). The reaction of the two cell lines to the recycling-processes were observed. The supernatant taken from the recycling phase of the fermentation and from the repeated batch, respectively, were fractionalised by gelfiltration. Each fraction was tested for inhibition using an activity bioassay (MTT) [2]." @default.
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- W12129628 date "1994-01-01" @default.
- W12129628 modified "2023-09-23" @default.
- W12129628 title "Inhibitory Substance(s) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line" @default.
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- W12129628 doi "https://doi.org/10.1016/b978-0-7506-1845-8.50042-9" @default.
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