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- W121720478 abstract "Columns for reversed-phase HPLC (RP-LC) can be characterized by five, retention-related parameters: H (hydrophobicity), S (steric selectivity), A (hydrogen-bond acidity), B (hydrogen-bond basicity), and C (cation-exchange behavior). In the present study, values of the latter parameters have been measured for 92 type-B (low metals content)alkyl-silica columns and compared to column properties such as ligand length,ligand concentration, pore diameter, and the presence or absence of end-capping. With the exception of five columns of unusual design, retention factors, k, for 16 representative test compounds were correlated with values of H, S, etc., within an average +/- 1.2% (1 standard deviation, SD), suggesting that all significant solute-column interactions are recognized by these five column parameters. A single-valued function F(s) is proposed to measure differences in selectivity for any two RP-LC columns whose values of H, S, etc., are known. This allows the easy selection of columns whose selectivity is desired to be either similar to or different from a starting column, for application in either routine analysis or method development." @default.
- W121720478 created "2016-06-24" @default.
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- W121720478 date "2003-06-01" @default.
- W121720478 modified "2023-10-17" @default.
- W121720478 title "Column selectivity in reversed-phase liquid chromatography" @default.
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- W121720478 doi "https://doi.org/10.1016/s0021-9673(03)00512-0" @default.
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