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- W121897060 abstract "The aim of this study was to evaluate new methods to improve detection and investigation of the effects of chronic or subclinical infection with Trypanosoma evansi in various mammalian species. Some of the more resistant host species, including pigs and buffaloes, are present in large feral populations in the northern parts of Australia, the area where T. evansi is most likely to gain entry to the country. Existing tests are not sufficiently reliable to detect all cases of disease and they cannot distinguish acute from chronic infections. Furthermore, the tests have different sensitivities in different host species. Surveillance for trypanosomiasis in Australia is problematic because of the need to work in remote parts of northern Australia where provision of a cold-chain for traditional blood and serum storage is difficult. An existing dried blood storage system was modified by treating cotton lint filter paper (Whatman #903) with a commercial post coating buffer (TropBio, Queensland). This treatment increased the longevity of antibodies to T. evansi in serum and blood stored on the paper (detected using an antibody-detection ELISA) compared to samples stored on plain paper, especially when the papers were stored under humid conditions and at high ambient temperatures. Attempts were made to improve the diagnostic utility and repeatability of antibody-ELISAs through the use of 2 recombinant T. brucei antigens (PFRA and GM6) and to optimize a competitive ELISA using RoTat 1.2 variable surface antigen and its monoclonal antibody. Antibody-detection using the two recombinant proteins was not sufficiently specific to enable their use for the detection of T. evansi. The RoTat 1.2 cELISA had good sensitivity and specificity (75% and 98% respectively) when used to test serum from cattle and buffaloes experimentally infected with T. evansi and uninfected animals. However, the test was not able to detect anti-T. evansi antibodies in serum from wallabies, pigs, a dog or a horse that were experimentally infected with T. evansi. The inability of the cELISA to detect anti-T. evansi antibodies may be due to the small number of samples tested or the lack of RoTat 1.2 specific antibodies in the animals tested. The feasibility of using an enzymatic test to detect trypanosome aminotransferase or antibodies to this enzyme was evaluated. Prior publications suggested that the detection of TAT was an appropriate diagnostic tool for the detection of T. evansi infection in camels. However, the results from this study did not support the use of this test for the detection of T. evansi infection in cattle or buffaloes with low to moderate parasitaemia. Trypanosomiasis is an immunological disease that affects most of the body’s organs, with more severe disease developing over time. Attempts were made to determine key cytokine and biochemical patterns that would distinguish infected from uninfected animals and acute from chronic infections. The results from this study showed that there was no specific pattern in serum cytokines or serum biochemistry that could be used to distinguish infected from uninfected animals, or different stages of disease. Immunohistochemistry was used on tissues from buffaloes and mice experimentally infected with T. evansi and T. brucei gambiense respectively to characterise the cellular immune response that was present. The immune response was predominantly cell mediated, with CD3+ T lymphocyte and macrophage infiltration occurring in most tissues. In end stage disease there was often suppression of the immune system with disruption of the architecture of the spleen and a decrease in B lymphocytes in the circulation. Trypanosomes were rarely visible in the tissues and were only seen in those animals with high parasitaemia. Lesions generally became more severe over time, but there was a large variation between animals, which suggests that immunohistochemistry is unsuitable as a diagnostic tool." @default.
- W121897060 created "2016-06-24" @default.
- W121897060 creator A5030285562 @default.
- W121897060 date "2009-01-01" @default.
- W121897060 modified "2023-09-27" @default.
- W121897060 title "Development of tools to improve the detection of Trypanosoma evansi in Australia" @default.
- W121897060 cites W1481611082 @default.
- W121897060 cites W1485500114 @default.
- W121897060 cites W1493102094 @default.
- W121897060 cites W1493836238 @default.
- W121897060 cites W1503200131 @default.
- W121897060 cites W1505423311 @default.
- W121897060 cites W1508518870 @default.
- W121897060 cites W1515531572 @default.
- W121897060 cites W1515615230 @default.
- W121897060 cites W1522823564 @default.
- W121897060 cites W1523963660 @default.
- W121897060 cites W1539460627 @default.
- W121897060 cites W1541183917 @default.
- W121897060 cites W1546877697 @default.
- W121897060 cites W1547565061 @default.
- W121897060 cites W1562277708 @default.
- W121897060 cites W1573976459 @default.
- W121897060 cites W1581633489 @default.
- W121897060 cites W1586392520 @default.
- W121897060 cites W1587121942 @default.
- W121897060 cites W1595687156 @default.
- W121897060 cites W1597728931 @default.
- W121897060 cites W1601925808 @default.
- W121897060 cites W1604151377 @default.
- W121897060 cites W1605840602 @default.
- W121897060 cites W1605967475 @default.
- W121897060 cites W1628094674 @default.
- W121897060 cites W1700762969 @default.
- W121897060 cites W1777081626 @default.
- W121897060 cites W1813742978 @default.
- W121897060 cites W181606021 @default.
- W121897060 cites W1822821191 @default.
- W121897060 cites W1832269131 @default.
- W121897060 cites W1853022482 @default.
- W121897060 cites W185783770 @default.
- W121897060 cites W1857990346 @default.
- W121897060 cites W1861660664 @default.
- W121897060 cites W1874303663 @default.
- W121897060 cites W1894873888 @default.
- W121897060 cites W1897978554 @default.
- W121897060 cites W1898585162 @default.
- W121897060 cites W1930352762 @default.
- W121897060 cites W194138430 @default.
- W121897060 cites W1942668068 @default.
- W121897060 cites W1945635812 @default.
- W121897060 cites W1959095578 @default.
- W121897060 cites W1966456095 @default.
- W121897060 cites W1968990454 @default.
- W121897060 cites W1969384419 @default.
- W121897060 cites W1970853569 @default.
- W121897060 cites W1971853331 @default.
- W121897060 cites W1971925374 @default.
- W121897060 cites W1972152422 @default.
- W121897060 cites W1974174801 @default.
- W121897060 cites W1974540113 @default.
- W121897060 cites W1975300155 @default.
- W121897060 cites W1977524538 @default.
- W121897060 cites W1979300132 @default.
- W121897060 cites W1979493672 @default.
- W121897060 cites W1980650947 @default.
- W121897060 cites W1980945951 @default.
- W121897060 cites W1980979493 @default.
- W121897060 cites W1982092413 @default.
- W121897060 cites W1985165897 @default.
- W121897060 cites W1985450391 @default.
- W121897060 cites W1986309700 @default.
- W121897060 cites W1986832558 @default.
- W121897060 cites W1987150557 @default.
- W121897060 cites W1987821415 @default.
- W121897060 cites W1988194793 @default.
- W121897060 cites W1990443993 @default.
- W121897060 cites W1991342293 @default.
- W121897060 cites W1992760440 @default.
- W121897060 cites W1993738226 @default.
- W121897060 cites W1994203972 @default.
- W121897060 cites W1994269537 @default.
- W121897060 cites W1997589622 @default.
- W121897060 cites W1998193498 @default.
- W121897060 cites W1998476079 @default.
- W121897060 cites W1999030993 @default.
- W121897060 cites W1999706120 @default.
- W121897060 cites W1999928494 @default.
- W121897060 cites W2000695448 @default.
- W121897060 cites W2000726781 @default.
- W121897060 cites W2002076761 @default.
- W121897060 cites W2002726220 @default.
- W121897060 cites W2002765315 @default.
- W121897060 cites W2003555587 @default.
- W121897060 cites W2003733069 @default.
- W121897060 cites W2004633550 @default.
- W121897060 cites W2004850525 @default.
- W121897060 cites W2005246059 @default.
- W121897060 cites W2005885384 @default.
- W121897060 cites W2006708761 @default.