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- W122096552 abstract "RONI NUGRAHA. Production of cellulase by Penicillium nalgiovense SS240 on Palm Oil Bunch. Under the direction of EMAN KUSTAMAN and TRESNAWATI PURWADARIA The enzymatic hydrolysis of cellulose requires the use of cellulase, a multiple enzyme system consisting of endo 1,4-β-D-glucanase, exo 1,4-β-D-glucanase and β-glucosidase. The production of microbial cellulase will be influenced by the intake of carbon and nitrogen. The optimal production of these enzymes by using P. nalgiovense SS240 was observed at different pretreated substrates and level of nitrogen. P. nalgiovense SS240 grown on palm oil bunch treated NaOH, non-treated palm oil bunch and pollard treated NaOH for each by submerged fermentation method to find the best carbon source for production of cellulose. Research continued to find the best nitrogen source for enzyme production. Nitrogen source varied that are ammonium sulphate + urea and sodium nitrate. The best nitrogen source then used for next stage of research that is optimisation of enzyme production by varied nitrogen level on medium as 0,017%, 0,034% and 0,068%. Saccharification on rice bran used as parameter for cellulase activities in all research stage. CMCase and β-glucosidase activity tested only in third stage of research. Experiment results showed that non-treated palm oil bunch yield better saccharification activity than palm oil bunch treated NaOH and pollard NaOH. The best nitrogen source showed by sodium nitrate with saccharification activity was 215,3 μmol/ml. Various nitrogen source didn’t give different significant level (p<0,05) toward cellulase activities. The highest CMCase, β-glucosidase and saccharification reached on nitrogen level 0.017% that 0,017% yaitu 0,027 U/ml, 0,046 U/ml and 172,4 μmol/ml respectively. PRODUKSI ENZIM SELULASE OLEH Penicillium nalgiovense SS240 PADA SUBSTRAT TANDAN SAWIT" @default.
- W122096552 created "2016-06-24" @default.
- W122096552 creator A5050218089 @default.
- W122096552 date "2006-01-01" @default.
- W122096552 modified "2023-09-27" @default.
- W122096552 title "Produksi Enzim Selulase oleh Penicillium nalgiovense SS240 pada Substrat Tandan Sawit" @default.
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