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- W122361210 abstract "The UVB (295-320 nm) component of sunlight was once thought to be the sole cause of photoaging and skin cancer. However, there is now compelling evidence to suggest that chronic irradiation with UVA (320-400 nm) is a significant component of the etiologies of these diseases. To identify acute markers of UVA damage, we investigated UVA-induced mutagenesis in vivo by using a lacI transgenic mouse mutation assay. The backs of adult female C57BL/6 Big Blue {reg_sign} mice were shaved and exposed daily to a low or a high dose of UVA for 5 consecutive days. One group remained unexposed. The high dose of UVA significantly increased the mutant frequency in skin determined 12 days after the last exposure. Mutant frequencies were (Avg {plus_minus} SEM, n=7-8/group): 6.1 {plus_minus} 0.5 x 10{sup {minus}5} (high dose). DNA sequence analysis of mutant lacI genes demonstrated that the high dose of UVA produced a different mutational spectrum compared to control. The mutational spectrum from the low dose mutants was not different from the control spectrum in skin generated previously; the predominant classes of recovered mutations were GC{yields}At transitions at CpG sites (11/35) and GC {yields}TA transversions (12/35). In contrast, in the high dose group, GC {yields}ATmore » transitions at non-CpG sites predominated (61/97 mutations); three tandem base substitutions (1 GG {yields}AA; 2 CC{r_arrow}TT) were uniquely recovered; and an increased frequency of recovered GC{yields}CG substitutions was observed (12/97 vs. none in controls). The recovered high dose spectrum is consistent with the types of DNA damage generated by UVA as well as by reactive oxygen species. These studies demonstrate that UVA is mutagenic in vivo and that this assay can be used to study early events in UVA-induced skin damage.« less" @default.
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- W122361210 date "1995-11-01" @default.
- W122361210 modified "2023-09-26" @default.
- W122361210 title "UVA-induced mutational spectra in the laci gene from transgenic mouse skin" @default.
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