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- W1225871036 abstract "Research Article1 September 1987free access Metal ion regulated gene expression: use of a plastocyanin-less mutant of Chlamydomonas reinhardtii to study the Cu(II)-dependent expression of cytochrome c-552. S. Merchant S. Merchant Biological Laboratories, Harvard University, Cambridge, MA 02138. Search for more papers by this author L. Bogorad L. Bogorad Biological Laboratories, Harvard University, Cambridge, MA 02138. Search for more papers by this author S. Merchant S. Merchant Biological Laboratories, Harvard University, Cambridge, MA 02138. Search for more papers by this author L. Bogorad L. Bogorad Biological Laboratories, Harvard University, Cambridge, MA 02138. Search for more papers by this author Author Information S. Merchant1 and L. Bogorad1 1Biological Laboratories, Harvard University, Cambridge, MA 02138. The EMBO Journal (1987)6:2531-2535https://doi.org/10.1002/j.1460-2075.1987.tb02540.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Ac-208, a plastocyanin-deficient strain of Chlamydomonas reinhardtii, lacks the mRNA for pre-apoplastocyanin although Southern analyses indicate no gross deletion in the single gene for pre-apoplastocyanin. The alternative electron carrier, cytochrome c-552, is expressed only in Cu(II)-deficient cells of the mutant. Thus, the mutant strain can grow photoautotrophically in Cu(II)-deficient but not in Cu(II)-sufficient medium. This indicates that the absence of plastocyanin is the only defect (with respect to photosynthesis) in ac-208. Phenotypic revertants of ac-208 are capable of photoautotrophic growth by virtue of constitutive [with respect to Cu(II)] synthesis of cytochrome c-552. The revertant phenotype is attributed to a decreased internal concentration of Cu(II). This indicates that the Cu(II)-dependent expression of cytochrome c-552 synthesis is affected directly by the concentration of Cu(II) rather than indirectly by the level of plastocyanin in the cell. Previous ArticleNext Article Volume 6Issue 91 September 1987In this issue RelatedDetailsLoading ..." @default.
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- W1225871036 title "Metal ion regulated gene expression: use of a plastocyanin-less mutant of Chlamydomonas reinhardtii to study the Cu(II)-dependent expression of cytochrome c-552." @default.
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