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- W1228640315 abstract "Bacillus subtilis is actively used for both biochemical and genetic experiments because of its prototrophic nature and the availability of genetic systems. The spore stage of Bacillus subtilis provides a simple model system for studying the problems of cell differentiation. The processes of sporulation, germination, and outgrowth can be largely synchronized under controlled conditions. This chapter reviews some of the techniques needed for study various biochemical problems using B. subtilis. Bacillus subtilis grows on antibiotic medium 3 (Penassay medium- Difco) with a doubling time of 20–25 minutes at 37°. It offers three systems amenable to mutagenic treatment—namely, spores, vegetative cells, and purified DNA. The use of purified DNA has the advantage that each mutant found represents an independent event when the cells are plated directly from the transformation mixture. Mutants defective in the processes of germination or sporulation are isolated by a variety of procedures, including heat treatment of spores and acridine orange treatment of vegetative cells. Various genetic techniques considered include transformation, transduction, and transfection. B. subtilis spores can be prepared on solid or liquid media. The nitrogen and carbon sources supplied influence the degree and rate of spore formation in B. subtilis." @default.
- W1228640315 created "2016-06-24" @default.
- W1228640315 creator A5011008130 @default.
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- W1228640315 creator A5040544306 @default.
- W1228640315 creator A5068641892 @default.
- W1228640315 creator A5069097750 @default.
- W1228640315 date "1970-01-01" @default.
- W1228640315 modified "2023-10-10" @default.
- W1228640315 title "[2] Experimental methods for Bacillus subtilis" @default.
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