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• W123076191 abstract "Maritalea sp. RANG is the first rhizobial isolate from the accessory nidamental gland of Euprymna scolopes. Presented here are features from a 3.4Mb assembly of its genome Methods, Results, Discussion The accessory nidamental gland (ANG) of cephalopods is dominated by Alphaproteobacteria. The ANG of Euprymna scolopes is no exception, with members of the Phaeobacter genus being the most abundant. While genomes of several of these have been sequenced, no genomes yet exist for the minority constituents of the ANG consortium, which include members of the Rhizobiales. The isolate Maritalea sp. RANG was isolated on a plate of Reasoner’s 2A agar supplemented with sea salts and potassium nitrate and incubated anaerobically. The full-length 16S gene was sequenced and compared with 454 metagenomic 16S fragments which showed >98% identity to several of the reads, suggesting that this isolate is representative of the Rhizobia population within the ANG. When compared to characterized isolates, it had 97.6% identity to Maritalea porphyrae, originally isolated from red algae (215) and was given the preliminary designation as a member of the Maritalea genus. The genome of Maritalea sp. RANG was sequenced by creating an Illumina Nextera-XT library and sequenced on a MiSeq Sequencer using 2 x 250 (Illumina, San Diego, CA). This resulted in 933, 244 reads which was assembled in to a 3.4 mB genome in 107 contigs using the CLC Genomic Workbench (CLC, Aarhus, Denmark) with approximately 68x coverage. 90% of the genome is contained in a single 3mB" @default.
• W123076191 created "2016-06-24" @default.
• W123076191 creator A5043107754 @default.
• W123076191 date "2014-01-01" @default.
• W123076191 modified "2023-09-24" @default.
• W123076191 title "Bacterial Symbioses and the Innate Immune Response of the Model Host: Euprymna scolopes" @default.
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