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• W123996942 abstract "This chapter discusses magnetite–protein conjugates for the separation of cells by high-gradient magnetic filtration. The use of magnetic forces for fractionation of cell subpopulations in a cell suspension requires a satisfactory method of selectively attaching a magnetic label to one cell type and an apparatus that can exert a physical force on and efficiently sequester the labeled cell population with minimal contamination by unlabeled cells. The strength of the magnetic force that a labeled cell will feel in an experimental apparatus is determined by two things: (1) the strength of the magnetization that an applied field can induce in the labeling material on the cell and (2) the gradient of the magnetic field at the location of the cell. The system has been demonstrated with several formulations of magnetic label, differing in biochemical details of the labeling process. These have been tested and compared, each in an appropriate modification of the same model leukocyte labeling and sorting system. That system is the labeling and removal of T lymphocytes from a suspension of mouse spleen cells. Increasing the magnetic contrast between tagged and bystander cells is being approached as a problem in minimizing uptake of colloidal magnetite by normal cells and one of maximizing the ligands on particles to improve specific uptake by antibody-tagged cells." @default.
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• W123996942 date "1987-01-01" @default.
• W123996942 modified "2023-10-10" @default.
• W123996942 title "Magnetite–Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration" @default.
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• W123996942 doi "https://doi.org/10.1016/b978-0-12-564504-1.50015-5" @default.
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