FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1241181863> ?p ?o ?g. }

• W1241181863 endingPage "271" @default.
• W1241181863 startingPage "259" @default.
• W1241181863 abstract "High concentrations of y-amino butyric acid (GABA) in blood did not result in an elevation of brain GABA levels. A barrier exists which restricts the passage of GABA from blood to nervous tissue. But recent histochemical studies on the distribution of GABA-a-keto-glutarate transaminase in the mammalian central nervous system suggested that the barrier may be in part a reflection of high GABA-transaminase activity in the walls of cerebral blood vessels and in the cells lining the cerebrospinal fluid spaces. To explore this suggestion further, the paper has a study that was conducted on the distribution of GABA-transaminase in kidney and liver. GABA appears to pass freely from blood to kidney, since as much as fifty per cent of the total GABA injected can be recovered from urine. Similarly, the concentration of GABA in liver after injection parallels that of the blood. The paper consists of the histochemical procedures for the localization of GABA-transaminase. The method is based on the fact that, in the course of the metabolism of GABA to succinic acid, NADH is formed, which in turn reduces a tetrazolium salt (Nitro BT) to its formazan form. Formazan is insoluble and will precipitate out at sites in the tissue where the combined presences of GABA-a-keto glutarate transaminase and succinic semialdehyde dehydrogenase have caused the conversion of GABA to succinic acid. When sections of mammalian brain are incubated with a transaminase medium, strong formazan precipitation occurs at sites where GABA-transaminase and succinic semialdehyde dehydrogenase are present in combination. Although the presence of both enzymes is a prerequisite for the reaction, recent studies suggested that the transamination step is rate limiting with respect to the histochemical demonstration of GABA metabolism. As a conclusion, the paper states that the ependymal cell layer which lines the cerebrospinal fluid spaces is among the sites in the central nervous system which exhibit strong GABA-transaminase activity. The paper also has a brief on the specificity of the histochemical reaction for GABA. In order to determine the specificity of the histochemical reaction for GABA transaminase in kidney, hydrazinopropionic acid (15 mg/kg) was injected into a mouse and its kidney was sectioned two hours later. Such sections, when incubated in the transaminase medium, were identical in appearance to normal sections which had been incubated in a control medium lacking GABA." @default.
• W1241181863 created "2016-06-24" @default.
• W1241181863 creator A5015942308 @default.
• W1241181863 date "1968-01-01" @default.
• W1241181863 modified "2023-10-01" @default.
• W1241181863 title "A Possible Enzyme Barrier for γ-Aminobutyric Acid in the Central Nervous System" @default.
• W1241181863 cites W150129925 @default.
• W1241181863 cites W1997787389 @default.
• W1241181863 cites W2004917924 @default.
• W1241181863 cites W2034086886 @default.
• W1241181863 cites W2035763754 @default.
• W1241181863 cites W2054819470 @default.
• W1241181863 cites W2066675934 @default.
• W1241181863 cites W2088637181 @default.
• W1241181863 cites W2098133556 @default.
• W1241181863 cites W2130190854 @default.
• W1241181863 cites W2150693059 @default.
• W1241181863 cites W22874719 @default.
• W1241181863 doi "https://doi.org/10.1016/s0079-6123(08)64161-8" @default.
• W1241181863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5735305" @default.
• W1241181863 hasPublicationYear "1968" @default.
• W1241181863 type Work @default.
• W1241181863 sameAs 1241181863 @default.
• W1241181863 citedByCount "48" @default.
• W1241181863 countsByYear W12411818632013 @default.
• W1241181863 countsByYear W12411818632016 @default.
• W1241181863 countsByYear W12411818632022 @default.
• W1241181863 crossrefType "book-chapter" @default.
• W1241181863 hasAuthorship W1241181863A5015942308 @default.
• W1241181863 hasConcept C126322002 @default.
• W1241181863 hasConcept C134018914 @default.
• W1241181863 hasConcept C169760540 @default.
• W1241181863 hasConcept C181199279 @default.
• W1241181863 hasConcept C185592680 @default.
• W1241181863 hasConcept C2776877979 @default.
• W1241181863 hasConcept C2776892249 @default.
• W1241181863 hasConcept C2777421860 @default.
• W1241181863 hasConcept C2780091579 @default.
• W1241181863 hasConcept C529278444 @default.
• W1241181863 hasConcept C545706735 @default.
• W1241181863 hasConcept C55493867 @default.
• W1241181863 hasConcept C71924100 @default.
• W1241181863 hasConcept C86803240 @default.
• W1241181863 hasConcept C9497952 @default.
• W1241181863 hasConceptScore W1241181863C126322002 @default.
• W1241181863 hasConceptScore W1241181863C134018914 @default.
• W1241181863 hasConceptScore W1241181863C169760540 @default.
• W1241181863 hasConceptScore W1241181863C181199279 @default.
• W1241181863 hasConceptScore W1241181863C185592680 @default.
• W1241181863 hasConceptScore W1241181863C2776877979 @default.
• W1241181863 hasConceptScore W1241181863C2776892249 @default.
• W1241181863 hasConceptScore W1241181863C2777421860 @default.
• W1241181863 hasConceptScore W1241181863C2780091579 @default.
• W1241181863 hasConceptScore W1241181863C529278444 @default.
• W1241181863 hasConceptScore W1241181863C545706735 @default.
• W1241181863 hasConceptScore W1241181863C55493867 @default.
• W1241181863 hasConceptScore W1241181863C71924100 @default.
• W1241181863 hasConceptScore W1241181863C86803240 @default.
• W1241181863 hasConceptScore W1241181863C9497952 @default.
• W1241181863 hasLocation W12411818631 @default.
• W1241181863 hasLocation W12411818632 @default.
• W1241181863 hasOpenAccess W1241181863 @default.
• W1241181863 hasPrimaryLocation W12411818631 @default.
• W1241181863 hasRelatedWork W1980339709 @default.
• W1241181863 hasRelatedWork W2020959757 @default.
• W1241181863 hasRelatedWork W2042033030 @default.
• W1241181863 hasRelatedWork W2044943066 @default.
• W1241181863 hasRelatedWork W2070511355 @default.
• W1241181863 hasRelatedWork W2082456910 @default.
• W1241181863 hasRelatedWork W2098742882 @default.
• W1241181863 hasRelatedWork W2127085070 @default.
• W1241181863 hasRelatedWork W2400445066 @default.
• W1241181863 hasRelatedWork W4233814061 @default.
• W1241181863 isParatext "false" @default.
• W1241181863 isRetracted "false" @default.
• W1241181863 magId "1241181863" @default.
• W1241181863 workType "book-chapter" @default.