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• W1244925421 abstract "Research Article1 December 1984free access Human globin gene transcription in injected Xenopus oocytes: enhancement by sodium butyrate. G.A. Partington G.A. Partington Search for more papers by this author N.J. Yarwood N.J. Yarwood Search for more papers by this author T.R. Rutherford T.R. Rutherford Search for more papers by this author G.A. Partington G.A. Partington Search for more papers by this author N.J. Yarwood N.J. Yarwood Search for more papers by this author T.R. Rutherford T.R. Rutherford Search for more papers by this author Author Information G.A. Partington, N.J. Yarwood and T.R. Rutherford The EMBO Journal (1984)3:2787-2792https://doi.org/10.1002/j.1460-2075.1984.tb02210.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have studied the expression of the human adult (beta) and foetal (gamma) globin genes by injecting a cosmid containing the complete -G gamma-A gamma-psi beta-delta-beta cluster into Xenopus oocytes. The transcripts from the gamma and beta genes were characterised with respect to their 5′ termini by S1 nuclease mapping using probes which extend 5′ to the cap site and 3′ into the first exon. The only beta transcripts detectable in injected oocytes spanned from either -231 or -177 (cap site +1) into the first exon. The sensitivity of this transcription to inhibition by a low concentration of alpha-amanitin indicated that polymerase II was responsible. S1 analysis of gamma gene transcripts showed that the predominant transcript mapped from a site at -227, but a low level of transcription from the cap site was also detectable. Incubation of oocytes in saline solution containing sodium butyrate prior to injection resulted in a significant stimulation of transcription from the gamma gene cap site. In contrast, transcription from the beta gene upstream sites was only slightly increased. The possible role of sodium butyrate in promoting this effect is discussed in the context of its known property as a modifier of cellular chromatin. Previous ArticleNext Article Volume 3Issue 121 December 1984In this issue RelatedDetailsLoading ..." @default.
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• W1244925421 date "1984-12-01" @default.
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• W1244925421 title "Human globin gene transcription in injected Xenopus oocytes: enhancement by sodium butyrate." @default.
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• W1244925421 doi "https://doi.org/10.1002/j.1460-2075.1984.tb02210.x" @default.
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