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• W12462873 abstract "Mutagenesis by the overlap extension PCR has become a standard method of creating mutations including substitutions, insertions, and deletions. Nonetheless, the established overlap PCR mutagenesis is limited in many respects. In particular, it has been difficult to make an insertion larger than 30 nt, since all sequence alterations must be embedded within the primer. Here, we describe a rapid and efficient method for creating insertions or deletions of any length at any position in a DNA molecule. This method is generally applicable, and therefore represents a significant improvement to the now widely used overlap extension PCR method." @default.
• W12462873 created "2016-06-24" @default.
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• W12462873 date "2010-01-01" @default.
• W12462873 modified "2023-10-12" @default.
• W12462873 title "Insertion and Deletion Mutagenesis by Overlap Extension PCR" @default.
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• W12462873 doi "https://doi.org/10.1007/978-1-60761-652-8_10" @default.
• W12462873 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20676981" @default.
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