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- W1246554611 abstract "Publisher Summary Glycomics is an emerging field in the post-genome/proteome era directed towards structural identification and elucidation of glycan functions in particular biological phenomena. The phenomena include cell–cell interaction events like differentiation, development, morphogenesis, embryogenesis, immunity, infection, tumorigenesis, and metastasis. This chapter describes recent advances and emerging techniques in lectin-based glycotechnologies. There are four key technologies available for glycan profiling. The first method is multi-dimensional liquid chromatography established for neutral N -linked glycans as two-dimensional mapping and for those including acidic glycans as three-dimensional mapping. The second technique is mass spectrometry (MS), which is a powerful aspect of advanced biotechnology. It is highly useful for both confirmation and estimation of glycan structures, including glycosaminoglycans. The third approach, capillary electrophoresis is based on a different separation principle from the rest. It exhibits advantages in speed and sensitivity and is used together with MS, predominantly for analytical purposes. The final approach, lectin microarray, is an emerging technique extensively supported by fundamental information provided by frontal affinity chromatography. It employs the use of special tools called lectins for structural analysis of glycan detection and purification in various research areas. These chromatographic and mass spectrometric techniques are necessary for structural studies of glycans as well as other analytical methods for elucidation of glycan biological functions. They provide an approach to the analysis of evolutionary issues involving comparative glycomics." @default.
- W1246554611 created "2016-06-24" @default.
- W1246554611 creator A5002952594 @default.
- W1246554611 date "2010-01-01" @default.
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- W1246554611 title "Chromatographic and Mass Spectrometric Techniques" @default.
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- W1246554611 doi "https://doi.org/10.1016/b978-0-12-373600-0.00007-x" @default.
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