FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W125294391> ?p ?o ?g. }

• W125294391 endingPage "387" @default.
• W125294391 startingPage "379" @default.
• W125294391 abstract "It is well established that liver microsomal cytochrome P-450 participates in steroid metabolism and probably also in the metabolism of anti-oestrogens such as tamoxifen (Nolvadex). Thus it is possible that variations in cytochrome P-450 levels may influence the responsiveness of human breast and endometrial carcinomas to endocrine therapy. Therefore a simple sensitive spectrophotometric assay for determining levels of cytochrome P-450-dependent cyclohexane hydroxylation activity in breast and uterine microsomes (microsomal fractions) has been developed. Cyclohexane was chosen as a substrate because of the relatively high levels of cyclohexane hydroxylase activity in tumour microsomes and because cyclohexane serves as a substrate for several forms of cytochrome P-450. As previously described [Senler, Dean, Pierce & Wittliff (1985) Anal. Biochem. 144, 152-158], a direct method utilizing isotope-dilution/gas chromatography-mass spectrometry was also developed in order to confirm the results of the spectrophotometric assay. The average activity (cyclohexane-dependent NADPH oxidation) for 139 human breast-tumour microsome preparations was 1.34 nmol/min per mg, which is in the range of that found in untreated mammalian liver (1-3 nmol/min per mg). Also, high enzyme activity was demonstrated in human ovary, normal uterus as well as uterine leiomyomas. Endocrine status appeared to influence enzyme levels, in that mammary tissue from virgin rats contained significantly (P less than 0.025) higher amounts of activity than did tissues from either pregnant or lactating rats. Furthermore, carbon monoxide, as well as an antibody against rat liver cytochrome P-450, completely inhibited NADPH oxidation by breast-carcinoma microsomes. These results strengthen our hypothesis that tumours with high levels of cytochrome P-450 may have a reduced response to additive endocrine therapy." @default.
• W125294391 created "2016-06-24" @default.
• W125294391 creator A5029079839 @default.
• W125294391 creator A5046186471 @default.
• W125294391 creator A5058354088 @default.
• W125294391 creator A5072346036 @default.
• W125294391 date "1985-04-15" @default.
• W125294391 modified "2023-09-27" @default.
• W125294391 title "Quantification of cytochrome P-450-dependent cyclohexane hydroxylase activity in normal and neoplastic reproductive tissues" @default.
• W125294391 cites W100346553 @default.
• W125294391 cites W126408028 @default.
• W125294391 cites W1484075233 @default.
• W125294391 cites W1498142637 @default.
• W125294391 cites W1499517824 @default.
• W125294391 cites W1504804986 @default.
• W125294391 cites W1507194289 @default.
• W125294391 cites W1532212858 @default.
• W125294391 cites W1550563358 @default.
• W125294391 cites W1673905022 @default.
• W125294391 cites W1775749144 @default.
• W125294391 cites W1967660683 @default.
• W125294391 cites W1970398376 @default.
• W125294391 cites W1988617504 @default.
• W125294391 cites W2004101290 @default.
• W125294391 cites W2018289835 @default.
• W125294391 cites W2034211968 @default.
• W125294391 cites W2051536602 @default.
• W125294391 cites W2068286076 @default.
• W125294391 cites W2073760781 @default.
• W125294391 cites W2074368593 @default.
• W125294391 cites W2079432673 @default.
• W125294391 cites W2100837269 @default.
• W125294391 cites W2125777038 @default.
• W125294391 cites W2155386297 @default.
• W125294391 cites W2158281093 @default.
• W125294391 cites W2324301924 @default.
• W125294391 cites W2406604379 @default.
• W125294391 cites W2406630756 @default.
• W125294391 cites W2418916358 @default.
• W125294391 cites W73811941 @default.
• W125294391 cites W946056603 @default.
• W125294391 doi "https://doi.org/10.1042/bj2270379" @default.
• W125294391 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1144856" @default.
• W125294391 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4004771" @default.
• W125294391 hasPublicationYear "1985" @default.
• W125294391 type Work @default.
• W125294391 sameAs 125294391 @default.
• W125294391 citedByCount "11" @default.
• W125294391 countsByYear W1252943912012 @default.
• W125294391 countsByYear W1252943912013 @default.
• W125294391 countsByYear W1252943912015 @default.
• W125294391 countsByYear W1252943912016 @default.
• W125294391 crossrefType "journal-article" @default.
• W125294391 hasAuthorship W125294391A5029079839 @default.
• W125294391 hasAuthorship W125294391A5046186471 @default.
• W125294391 hasAuthorship W125294391A5058354088 @default.
• W125294391 hasAuthorship W125294391A5072346036 @default.
• W125294391 hasBestOaLocation W1252943912 @default.
• W125294391 hasConcept C126322002 @default.
• W125294391 hasConcept C134018914 @default.
• W125294391 hasConcept C181199279 @default.
• W125294391 hasConcept C185592680 @default.
• W125294391 hasConcept C2780768313 @default.
• W125294391 hasConcept C2781109383 @default.
• W125294391 hasConcept C526171541 @default.
• W125294391 hasConcept C55493867 @default.
• W125294391 hasConcept C62231903 @default.
• W125294391 hasConcept C71924100 @default.
• W125294391 hasConcept C86803240 @default.
• W125294391 hasConcept C87644729 @default.
• W125294391 hasConceptScore W125294391C126322002 @default.
• W125294391 hasConceptScore W125294391C134018914 @default.
• W125294391 hasConceptScore W125294391C181199279 @default.
• W125294391 hasConceptScore W125294391C185592680 @default.
• W125294391 hasConceptScore W125294391C2780768313 @default.
• W125294391 hasConceptScore W125294391C2781109383 @default.
• W125294391 hasConceptScore W125294391C526171541 @default.
• W125294391 hasConceptScore W125294391C55493867 @default.
• W125294391 hasConceptScore W125294391C62231903 @default.
• W125294391 hasConceptScore W125294391C71924100 @default.
• W125294391 hasConceptScore W125294391C86803240 @default.
• W125294391 hasConceptScore W125294391C87644729 @default.
• W125294391 hasIssue "2" @default.
• W125294391 hasLocation W1252943911 @default.
• W125294391 hasLocation W1252943912 @default.
• W125294391 hasLocation W1252943913 @default.
• W125294391 hasLocation W1252943914 @default.
• W125294391 hasOpenAccess W125294391 @default.
• W125294391 hasPrimaryLocation W1252943911 @default.
• W125294391 hasRelatedWork W166915932 @default.
• W125294391 hasRelatedWork W1815932096 @default.
• W125294391 hasRelatedWork W1992900791 @default.
• W125294391 hasRelatedWork W2048342700 @default.
• W125294391 hasRelatedWork W2074076166 @default.
• W125294391 hasRelatedWork W2168838782 @default.
• W125294391 hasRelatedWork W2400024976 @default.
• W125294391 hasRelatedWork W2419414102 @default.
• W125294391 hasRelatedWork W4250393268 @default.

• next