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- W1255338587 abstract "Publisher Summary This chapter presents the methods that have devised for the production of deuterated Dictyostelium . The method for isolating actin from deuterated Dictyostelium is described as an example for the preparation of deuterated proteins from a eukaryote. The solutions used for extraction and purification can be prepared in H 2 O, as only exchangeable sites will lose deuterium and these are redeuterated by adding D 2 O to the purified protein. The chapter described a procedures using 50–75 g of washed amoebae that are obtained from 5 liters of culture medium. The production of deuterated Dictyostelium represents a major advance in the supply of deuterated eukaryotic macromolecules for structural studies. The procedure outlined is both cost-and time-efficient. D 2 O is the only source of deuterium that must be purchased, and this is recycled. Both E. coli and Dictyostelium readily grow on deuterated medium without need for adaptation and with generation times which are only about twice those on comparable protonated medium. With appropriate modification this procedure should be capable of producing a variety of deuterated macromolecules from Dictyostelium ." @default.
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- W1255338587 date "1987-01-01" @default.
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- W1255338587 title "Chapter 12 Preparation of Deuterated Actin from Dictyostelium discoideum" @default.
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- W1255338587 doi "https://doi.org/10.1016/s0091-679x(08)61647-7" @default.
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