Matches in SemOpenAlex for { <https://semopenalex.org/work/W1255589> ?p ?o ?g. }
- W1255589 abstract "The molecular biology of desiccation tolerance was investigated in the cyanobacteria with emphasis on Nostoc commune. Analysis of DNA from 41 samples of desiccated Nostoc spp. of varied age and global distribution led to the amplification of 43 independent tRNA(UAA) group 1 intron sequences. Phylogenetic analysis of the entire data set made it possible to define the form species Nostoc commune. The synthase (spsA) and phosphatase (sppA) genes required for the synthesis of sucrose were isolated from cyanobacterium Synechocystis sp. strain PCC 6803 and overexpressed in E. coli in two different vector constructions. Transformants had a marked increased capacity for desiccation tolerance. Sucrose synthesis was confirmed through thin layer chromatography (TLC) analysis of cell extracts from transformants. Long-term stability of DNA in desiccated Nostoc samples was demonstrated by the ability to amplify selected gene loci from samples stored dry for decades. Successful amplification in some samples was possible only after treatment with phenacylthiazolium bromide, a reagent that disrupts covalent cross-links; indicating that the DNA was modified by cross-links that occurred between reducing sugars and the primary amines on the DNA. Abundant superoxide dismutase was released following rehydration of desiccated field material N. commune CHEN after 13 years in the dry state. sodF mRNA was present in the dry material but was turned over within 15 min of rehydration. mRNA levels then rose and appeared to reach steady state levels after 3 hours and remained abundant after 24 hours of rehydration." @default.
- W1255589 created "2016-06-24" @default.
- W1255589 creator A5084801102 @default.
- W1255589 date "2003-03-11" @default.
- W1255589 modified "2023-09-27" @default.
- W1255589 title "Molecular Biology of Desiccation Tolerance in the Cyanobacterium Nostoc commune" @default.
- W1255589 cites W1487100945 @default.
- W1255589 cites W1489408430 @default.
- W1255589 cites W1491876634 @default.
- W1255589 cites W1501748607 @default.
- W1255589 cites W1502542469 @default.
- W1255589 cites W1517069530 @default.
- W1255589 cites W1519185585 @default.
- W1255589 cites W1526731452 @default.
- W1255589 cites W1535142704 @default.
- W1255589 cites W1540484186 @default.
- W1255589 cites W1562498242 @default.
- W1255589 cites W1598778974 @default.
- W1255589 cites W1626710467 @default.
- W1255589 cites W1713353975 @default.
- W1255589 cites W171461912 @default.
- W1255589 cites W1716204645 @default.
- W1255589 cites W1734082729 @default.
- W1255589 cites W1755532834 @default.
- W1255589 cites W1789666962 @default.
- W1255589 cites W1821152284 @default.
- W1255589 cites W1834656874 @default.
- W1255589 cites W1848557 @default.
- W1255589 cites W1909988822 @default.
- W1255589 cites W1931878801 @default.
- W1255589 cites W1963107166 @default.
- W1255589 cites W1965248169 @default.
- W1255589 cites W1965396828 @default.
- W1255589 cites W196739373 @default.
- W1255589 cites W1967676653 @default.
- W1255589 cites W1969097369 @default.
- W1255589 cites W1969484170 @default.
- W1255589 cites W1971165347 @default.
- W1255589 cites W1975553694 @default.
- W1255589 cites W1981830941 @default.
- W1255589 cites W1982778809 @default.
- W1255589 cites W1982870743 @default.
- W1255589 cites W1983233459 @default.
- W1255589 cites W1983786986 @default.
- W1255589 cites W1984352800 @default.
- W1255589 cites W1989952092 @default.
- W1255589 cites W1994123472 @default.
- W1255589 cites W1994947012 @default.
- W1255589 cites W1996258759 @default.
- W1255589 cites W1998381553 @default.
- W1255589 cites W2000223285 @default.
- W1255589 cites W2001282030 @default.
- W1255589 cites W2009290849 @default.
- W1255589 cites W2010877168 @default.
- W1255589 cites W2011803829 @default.
- W1255589 cites W2016572584 @default.
- W1255589 cites W2017626786 @default.
- W1255589 cites W2019977292 @default.
- W1255589 cites W2021398206 @default.
- W1255589 cites W2025748287 @default.
- W1255589 cites W2026382071 @default.
- W1255589 cites W2030966943 @default.
- W1255589 cites W2035252235 @default.
- W1255589 cites W2039568284 @default.
- W1255589 cites W2040951800 @default.
- W1255589 cites W2045972444 @default.
- W1255589 cites W2048575968 @default.
- W1255589 cites W2049820913 @default.
- W1255589 cites W2051019730 @default.
- W1255589 cites W2052367587 @default.
- W1255589 cites W2056031907 @default.
- W1255589 cites W2056180623 @default.
- W1255589 cites W2059246521 @default.
- W1255589 cites W2060823909 @default.
- W1255589 cites W2063479670 @default.
- W1255589 cites W2065461553 @default.
- W1255589 cites W2066933820 @default.
- W1255589 cites W2068469369 @default.
- W1255589 cites W2068687524 @default.
- W1255589 cites W2069668852 @default.
- W1255589 cites W2070625410 @default.
- W1255589 cites W2075185730 @default.
- W1255589 cites W2075306011 @default.
- W1255589 cites W2076161798 @default.
- W1255589 cites W2079568673 @default.
- W1255589 cites W2079734908 @default.
- W1255589 cites W2080791625 @default.
- W1255589 cites W2083119773 @default.
- W1255589 cites W2090324283 @default.
- W1255589 cites W2091220428 @default.
- W1255589 cites W2092916361 @default.
- W1255589 cites W2097571219 @default.
- W1255589 cites W2097706568 @default.
- W1255589 cites W2103382953 @default.
- W1255589 cites W2107919301 @default.
- W1255589 cites W2107941964 @default.
- W1255589 cites W2108605550 @default.
- W1255589 cites W2112389572 @default.
- W1255589 cites W2112768570 @default.
- W1255589 cites W2113007696 @default.