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- W1256571036 abstract "Animal cells maintained in cell culture in the logarithmic phase of growth provide a ready source of rapidly dividing cells. By the addition of a mitotic inhibitor to these cultures, it is possible to obtain many of the cells in metaphase arrest. This chapter describes the procedures that have been used in laboratory to isolate metaphase chromosomes from these cultures.. The procedure that has been used to obtain distinctive size groups of metaphase Chinese hamster chromosomes is also described. These studies were carried out with cells maintained in suspension culture. This method of cell propagation facilitates the accumulation of large numbers of cells in metaphase arrest. The fractionated chromosomes that are currently available should prove valuable as a source of three distinct genetic classes of DNA. It is hoped that the assignment of gene loci to these chromosome groups can be made using hybridization techniques. Vinblastine sulfate has been used to arrest cells at metaphase. The chapter illustrates the percentage of HeLa cells accumulated in metaphase at different times after the addition of vinblastine." @default.
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- W1256571036 date "1969-01-01" @default.
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- W1256571036 title "Chapter 12 Isolation and Fractionation of Metaphase Chromosomes" @default.
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- W1256571036 doi "https://doi.org/10.1016/s0091-679x(08)62123-8" @default.
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