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- W1258321623 abstract "This chapter discusses the isolation, characterization, and detection of osteocalcin. Osteocalcin is a small protein (5800 daltons) comprising 10-20% of the noncollagenous protein in the bone. Because osteocalcin is tightly adsorbed to the hydroxyapatite mineral phase of the bone, thorough extraction is best achieved by dissolving the mineral of finely pulverized bone. Hydroxyapatite is soluble in neutral 0.5M ethylenediaminetetraacetic acid (EDTA), as well as in a variety of mineral and organic acids. Uniformly high yields of 1-2 mg of osteocalcin per gram of dry bone are obtained with EDTA, whereas acid procedures often leave 10-25% of the protein behind, presumably because of precipitation during excursions through the osteocalcin isoelectric point. The use of radioimmunoassay for the measurement of osteocalcin offers the advantages of specificity, sensitivity, and technical simplicity. The method can easily detect nanogram quantities of the protein in bone extracts, cell cultures, and serum. The assay is based on the competition of the radioactively labeled antigen and an identical nonlabeled antigen for binding to a specific antibody. The amount of labeled antigen bound to the antibody is inversely proportional to the amount of unlabeled antigen present in the system." @default.
- W1258321623 created "2016-06-24" @default.
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- W1258321623 creator A5044743272 @default.
- W1258321623 creator A5078993249 @default.
- W1258321623 date "1984-01-01" @default.
- W1258321623 modified "2023-10-13" @default.
- W1258321623 title "Osteocalcin: Isolation, characterization, and detection" @default.
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- W1258321623 doi "https://doi.org/10.1016/0076-6879(84)07036-1" @default.
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