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- W1265675145 abstract "逆转录 聚合酶链反应 (ＲＴ ＰＣＲ)检测慢性粒细胞白血病(慢粒 )ｂｃｒ ａｂｌｍＲＮＡ已应用于临床诊断和微小残留病的检测 ,但该方法不能反映ｂｃｒ ａｂｌｍＲＮＡ和细胞结构之间的关系 ,且ＲＮＡ提取操作要求较高 ,应用受到一定的限制。我们拟建立直接原位ＲＴ ＰＣＲ检测慢粒ｂｃｒ ａｂｌｍＲＮＡ的方法。材料和方法1 细胞 抽取经临床确诊的慢粒患者外周静脉血 ,Ｆｉｃｏｌｌ分离出外周血单个核细胞。2 细胞涂片及固定 取 2× 10 6 /ｍｌ单个核细胞悬液 5 0 μｌ,离心涂片于包被 0 .2ｇ Ｌ多聚赖氨酸的载片上 ,用体积分数为4%的多聚甲醛固定 2 0ｍｉｎ。3 细胞预处理 10 μ" @default.
- W1265675145 created "2016-06-24" @default.
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- W1265675145 date "2000-07-14" @default.
- W1265675145 modified "2023-09-23" @default.
- W1265675145 title "直接原位逆转录—聚合酶链反应检测慢性粒细胞白血病bcr／abl mRNA" @default.
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