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- W1266708524 abstract "Two pairs of primers were designed to amplify the gene fragments encoding muramidase-released protein(MRP) and fibrinogen-binding protein(FBP) of Streptococcus suis type 2(SS2).The recombinant expression plasmids pET32a-mrp,pET32a-fbp and pET32a-mrp-fbp were respectively constructed with the PCR-amplified fragments of mrp and fbp genes,and then transformed into Escherichia coli BL21(DE3) induced by IPTG.SDS-PAGE analysis showed that the expressed MRP,FBP and MRP-FBP was 47 ku,52 ku and 80 ku in molecular mass respectively.Western-blot analysis showed that MRP-FBP could be recognized by the serum against SS2 whole cell.Adhesin vaccines were prepared respectively with MRP,FBP and MRP-FBP as antigen.To determine protective efficacy of the three vaccines,the vaccine with MRP-FBP could protect 80% of the mice to survive by the test of mice vaccination.It was concluded that the recombinant MRP-FBP was the best candidate antigen for the development of subunit vaccines against SS2 up to date." @default.
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- W1266708524 date "2009-01-01" @default.
- W1266708524 modified "2023-09-27" @default.
- W1266708524 title "Protective efficacy of the expressed recombinant MRP-FBP from Streptococcus suis type 2 in mice." @default.
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