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- W1274918937 abstract "legume-rhizobia symbioses, free-living rhizobia colonise root nodules anddevelop into N2 fixing specialists known as bacteroids. During bacteroiddevelopment, rhizobia must adapt to the nodule environment, consisting of reactiveoxygen species, low oxygen, antimicrobial secondary metabolites, low pH and insome nodules, antimicrobial peptides. This study offers a holistic insight into theprocesses required by R. leguminosarum during bacteroid development in nodulesformed on four legumes: Pisum sativum, Vicia faba, Vicia hirsuta and Phaseolusvulgaris.Initially, a high-throughput mutagenesis strategy was used to target genesupregulated during bacteroid development. Screening forty-two mutants on P.sativum identified some moderate phenotypes but more importantly, highlightedfunctional redundancy between certain gene products. A clear example of functionalredundancy was seen between the Mn2+ transporters SitABCD and MntH. Singlemutations in sitA or mntH did not cause a symbiotic phenotype whereas the doublemutant could not form bacteroids on P. sativum, V. faba or V. hirsuta. Intriguingly,no symbiotic phenotype for the double mutant was observed on P. vulgaris. Inaddition to Mn2+ transporters, a Mg2+ channel, MgtE, that is essential for growth inMg2+-limited medium at low pH was identified. As with the Mn2+ transporters, therequirement of MgtE during symbiosis depended upon the species of the hostlegume.Reasons for host-dependent requirement of SitABCD, MntH and MgtE arediscussed.The requirement of three O2-responsive regulators that govern regulatory pathwaysessential to N2 fixation was also investigated. FnrN appears to be the major O2-responsive regulator required for symbiosis but in addition to fnrN, two genes, fixLand fixLc, need to be mutated to prohibit N2 fixation. Other findings include aputative toxin-antitoxin system that hinders N2 fixation when disturbed." @default.
- W1274918937 created "2016-06-24" @default.
- W1274918937 creator A5018497752 @default.
- W1274918937 date "2013-09-01" @default.
- W1274918937 modified "2023-09-26" @default.
- W1274918937 title "Physiological response ofRhizobium leguminosarum during bacteroiddevelopment" @default.
- W1274918937 hasPublicationYear "2013" @default.
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