FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1276555013> ?p ?o ?g. }

• W1276555013 endingPage "20" @default.
• W1276555013 startingPage "11" @default.
• W1276555013 abstract "Recently, we described a new class of antisense oligonucleotides that can be used to direct the cleavage of mRNAs in Xenopus laevis embryos by RNase H (Dagle et al., Nucleic Acids Res. 18, 4751-4757). In this study, we have examined several factors that determine the activity of these derivatives. In embryos, oligodeoxyribonucleotides were found to be rapidly degraded by a 3' exonuclease. Modification of 3'-terminal phosphodiester linkages as phosphoramidates blocks this activity. The predominant sites of endonucleolytic cleavage within the embryo are localized close to the 5' termini demonstrating the necessity of multiply modifying phosphodiester linkages at each end of the molecule. A stretch of at least six consecutive phosphodiester linkages is required to form an effective substrate for Xenopus RNase H; mRNA degradation with an oligonucleotide containing fewer than six contiguous unmodified internucleoside linkages is greatly diminished. Injection of an anti-cyclin B oligonucleotide containing eight unmodified residues results in degradation of cyclin B mRNAs and subsequent inhibition of embryonic cell division. An oligonucleotide with the same sequence but containing four consecutive phosphodiesters has no observable effect on the cell cycle. This last observation suggests that, in Xenopus embryos, hybridization alone has a limited role, if any, in oligonucleotide-mediated inhibition of gene expression." @default.
• W1276555013 created "2016-06-24" @default.
• W1276555013 creator A5011921444 @default.
• W1276555013 creator A5027457245 @default.
• W1276555013 creator A5042544520 @default.
• W1276555013 date "1991-01-01" @default.
• W1276555013 modified "2023-10-08" @default.
• W1276555013 title "Pathways of Degradation and Mechanism of Action of Antisense Oligonucleotides in<i>Xenopus laevis</i>Embryos" @default.
• W1276555013 cites W1563545224 @default.
• W1276555013 cites W1998610836 @default.
• W1276555013 cites W2021509469 @default.
• W1276555013 cites W2027982726 @default.
• W1276555013 cites W2028094900 @default.
• W1276555013 cites W2028612120 @default.
• W1276555013 cites W2048990794 @default.
• W1276555013 cites W2050289195 @default.
• W1276555013 cites W2054281456 @default.
• W1276555013 cites W2063915751 @default.
• W1276555013 cites W2067037742 @default.
• W1276555013 cites W2070919272 @default.
• W1276555013 cites W2071186381 @default.
• W1276555013 cites W2076934212 @default.
• W1276555013 cites W2091557759 @default.
• W1276555013 cites W2096739990 @default.
• W1276555013 cites W2103431676 @default.
• W1276555013 cites W2110290847 @default.
• W1276555013 cites W2134812217 @default.
• W1276555013 cites W2138430171 @default.
• W1276555013 cites W2148320915 @default.
• W1276555013 cites W2157888763 @default.
• W1276555013 cites W2395591703 @default.
• W1276555013 cites W48777568 @default.
• W1276555013 doi "https://doi.org/10.1089/ard.1991.1.11" @default.
• W1276555013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1668307" @default.
• W1276555013 hasPublicationYear "1991" @default.
• W1276555013 type Work @default.
• W1276555013 sameAs 1276555013 @default.
• W1276555013 citedByCount "90" @default.
• W1276555013 countsByYear W12765550132012 @default.
• W1276555013 countsByYear W12765550132013 @default.
• W1276555013 countsByYear W12765550132015 @default.
• W1276555013 countsByYear W12765550132016 @default.
• W1276555013 countsByYear W12765550132017 @default.
• W1276555013 countsByYear W12765550132018 @default.
• W1276555013 countsByYear W12765550132019 @default.
• W1276555013 countsByYear W12765550132020 @default.
• W1276555013 countsByYear W12765550132021 @default.
• W1276555013 countsByYear W12765550132022 @default.
• W1276555013 crossrefType "journal-article" @default.
• W1276555013 hasAuthorship W1276555013A5011921444 @default.
• W1276555013 hasAuthorship W1276555013A5027457245 @default.
• W1276555013 hasAuthorship W1276555013A5042544520 @default.
• W1276555013 hasConcept C104317684 @default.
• W1276555013 hasConcept C10879258 @default.
• W1276555013 hasConcept C129312508 @default.
• W1276555013 hasConcept C151730666 @default.
• W1276555013 hasConcept C153911025 @default.
• W1276555013 hasConcept C175156509 @default.
• W1276555013 hasConcept C2777501222 @default.
• W1276555013 hasConcept C2779535977 @default.
• W1276555013 hasConcept C38441620 @default.
• W1276555013 hasConcept C43369102 @default.
• W1276555013 hasConcept C552990157 @default.
• W1276555013 hasConcept C55493867 @default.
• W1276555013 hasConcept C67705224 @default.
• W1276555013 hasConcept C69766542 @default.
• W1276555013 hasConcept C82381507 @default.
• W1276555013 hasConcept C86803240 @default.
• W1276555013 hasConcept C95444343 @default.
• W1276555013 hasConceptScore W1276555013C104317684 @default.
• W1276555013 hasConceptScore W1276555013C10879258 @default.
• W1276555013 hasConceptScore W1276555013C129312508 @default.
• W1276555013 hasConceptScore W1276555013C151730666 @default.
• W1276555013 hasConceptScore W1276555013C153911025 @default.
• W1276555013 hasConceptScore W1276555013C175156509 @default.
• W1276555013 hasConceptScore W1276555013C2777501222 @default.
• W1276555013 hasConceptScore W1276555013C2779535977 @default.
• W1276555013 hasConceptScore W1276555013C38441620 @default.
• W1276555013 hasConceptScore W1276555013C43369102 @default.
• W1276555013 hasConceptScore W1276555013C552990157 @default.
• W1276555013 hasConceptScore W1276555013C55493867 @default.
• W1276555013 hasConceptScore W1276555013C67705224 @default.
• W1276555013 hasConceptScore W1276555013C69766542 @default.
• W1276555013 hasConceptScore W1276555013C82381507 @default.
• W1276555013 hasConceptScore W1276555013C86803240 @default.
• W1276555013 hasConceptScore W1276555013C95444343 @default.
• W1276555013 hasIssue "1" @default.
• W1276555013 hasLocation W12765550131 @default.
• W1276555013 hasLocation W12765550132 @default.
• W1276555013 hasOpenAccess W1276555013 @default.
• W1276555013 hasPrimaryLocation W12765550131 @default.
• W1276555013 hasRelatedWork W2027821518 @default.
• W1276555013 hasRelatedWork W2032573377 @default.
• W1276555013 hasRelatedWork W2048091400 @default.
• W1276555013 hasRelatedWork W2094235341 @default.
• W1276555013 hasRelatedWork W2135050886 @default.
• W1276555013 hasRelatedWork W2157888763 @default.
• W1276555013 hasRelatedWork W2162332760 @default.

• next