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- W1277411782 abstract "Optimization of metal salts concentration (screened by Placket–Burman design) was carried out for alkaline phosphatase production by a halotolerant facultative alkaliphile Bacillus flexus FPB17 through Response surface methodology. Eleven metal salts including NaCl, MgCl2, MnCl2, CaCl2, CoCl2, CuCl2, FeCl3, KCl, ZnCl2, NH4Cl and Pb(NO3)2 were screened using Placket–Burman design criterion. Four salts viz. CaCl2, MgCl2, KCl and NaCl showed significant effect (p < 0.05) on alkaline phosphatase production. The metal salt concentrations were optimized by Central Composite Design, the most suitable metal salt composition being CaCl2 – 0.05 g%; MgCl2 – 0.05 g%; KCl – 0.01 g% and NaCl – 0.5 g%. At these optimum levels of metal salts, 239.58 U/ml alkaline phosphatase was produced. Selection and optimization of metal salts by Response surface methodology resulted in 1.84-fold increased yields of alkaline phosphatase." @default.
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- W1277411782 date "2013-01-01" @default.
- W1277411782 modified "2023-09-26" @default.
- W1277411782 title "Optimization of metal salt requirement for production of alkaline phosphatase by a halotolerant facultative alkaliphile Bacillus flexus FPB17 using response surface methodology" @default.
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