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- W127794211 abstract "Nitrogen-fixing root nodules represent strong carbon sinks. Sugar partitioning was studied in three different symbiotic systems, namely a legume, Medicago truncatula, and two actinorhizal plants, Casuarina glauca and Datisca glomerata. The expression levels of sucrose synthase (SuSy) and genes encoding sugar transporters were compared in roots and nodules. Legume and actinorhizal symbioses differ with respect to hexose transporter (HT) expression: induction of HT genes was found in nodules of actinorhizal plants Datisca and Casuarina, but HT expression levels in Medicago nodules were strongly reduced compared to roots. Expression levels of sucrose transporter (ST) genes in Medicago nodules were strongly reduced compared to roots.Enzyme activities of three invertase isoforms (vacuolar, apoplastic and cytosolic) and SuSy was determined for roots and nodules. The sum of apoplastic, cytosolic, and vacuolar invertase activities is strongly reduced in nodules compared to roots of all three symbiotic systems studied. The major sucrose-cleaving activity in Medicago and Datisca roots and nodules is represented by SuSy. Extracts from Datisca and Casuarina contain a single immunoreactive SuSy protein band of about 92 kDa. Medicago contains an additional, somewhat larger SuSy isoform in roots, but not in nodules. SuSy activities were similar in soluble protein fractions from both roots and nodules of Medicago, while in Datisca nodules they were lower than in roots.The profiles of soluble sugars analysed in roots and nodules. Two unknown sugars were isolated from Datisca and identified using biochemical methods, NMR and mass spectroscopy. They represented rutinose (α-L-rhamnopyranosyl-(1→6)-D-glucose) and methylrutinose (α-L-rhamnopyranosyl-(1→6)-1-O-methyl-β-D-glucose). Both were present in high amounts in roots, nodules and leaves of Datisca. Sucrose is the major sugar of roots and nodules from Medicago and Casuarina, while the major sugar of Datisca roots and nodules is rutinose.Full-size hexose transporter cDNAs were isolated from Medicago (MtHT) and Datisca (DgHT) nodules. Based on their amino acid sequence, the encoded proteins belonged to different classes of sugar transporters. The functional characterization of DgHT in a heterologous system (yeast) demonstrated that DgHT is a high affinity, energy-dependent monosaccharide transporter with broad substrate specificity, probably an H+-symporter.The implications of these data on sugar partitioning mechanisms are discussed." @default.
- W127794211 created "2016-06-24" @default.
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- W127794211 date "2003-02-28" @default.
- W127794211 modified "2023-09-24" @default.
- W127794211 title "Carbon partitioning in nitrogen-fixing root nodules" @default.
- W127794211 hasPublicationYear "2003" @default.
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