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- W1278687568 abstract "[目的]研究草鱼烂鳃病病原约氏黄杆菌芳香氨基酸代谢途径的合成基因aroA基因的全序列。[方法]以草鱼烂鳃病病原M165菌株基因组DNA为模板,分别利用5′和3′的3个特异性巢式引物与随机引物,通过热不对称交错PCR（TAIL-PCR）扩增菌株M165的aroA序列基因,并对其序列进行分析。[结果]电泳检测结果表明,扩增产物与预期扩增结果相符;测序结果分析表明,aroA基因序列全长1230bp,编码410个氨基酸。aroA蛋白序列与Flavobacterium johnsoniae的aroA蛋白质序列,具有高度同源性。[结论]TAIL-PCR方法为基因全序列的克隆提供了一种简便、高效的方法。aroA基因全序列的获得为进一步阐明aroA等营养相关因子对鱼类烂鳃病病原的致病力的影响奠定基础。" @default.
- W1278687568 created "2016-06-24" @default.
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- W1278687568 date "2010-01-01" @default.
- W1278687568 modified "2023-09-25" @default.
- W1278687568 title "TAIL-PCR方法克隆约氏黄杆菌aroA基因序列（摘要）" @default.
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