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• W12793407 abstract "RONI RIDWAN. Effectivity of Silage and Probiotic on Rumen Ecology of Peranakan Ongole Cattle by Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis. Under direction of SRI BUDIARTI and ACHMAD DINOTO. Silages and Probiotics additives were used for improvement of cattle productivity. Key of effectivity of silage and probiotics was changed observation direct or indirect roles on rumen ecology. The objective of this study was to examine the effects of silage and probiotics on ruminal ecosystems in vivo using molecular approach. Terminal-restriction fragment length polymorphism (T-RFLP) analysis was used to detect changes of ecology communities based on 16S ribosomal deoxyribonucleic acid (16S rDNA). Two rumen canulated PO cattle were fed several diet ie; (R0) basal diet dry matter basis (Pennisetum purpureum 70% and commercial concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus plantarum BTCC570), (R2) silage + probiotics (Leuconostoc sp. BTCC531), (R3) Basal diet + probiotics (Leuconostoc sp. BTCC531). Digesta samples were colected 3 h after feeding for cellulolytic activities (DNS method), pH, parsial volatile fatty acids measurements (Gas Chromatography method), and T-RFLP analysis. T-RFLP analysis was performed using the 16S rDNA amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI, HaeIII and MspI. Result showed that effectivities of silage and probiotics given together of index Smith and Wilson evenness applied to T-RFLP ecology data (Evar) 0.89±0.04 was the highest. It was significantly (p<0.05) increased rumen metabolism product (%mM) such propionic acid (19.44), valeric acid (0.76), isobutyric acid (2.21), isovaleric acid (1.77) compared with basal diet. T-RFLP analysis has a potency to be used for comparisons of complex bacterial communities, especialy to detect changes in community structure in respons to diferent variabels and showed rumen bacteria diversity in the rumen." @default.
• W12793407 created "2016-06-24" @default.
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• W12793407 date "2009-01-01" @default.
• W12793407 modified "2023-09-23" @default.
• W12793407 title "Efektivitas Pemberian Silase dan Probiotik Terhadap Ekologi Rumen Sapi Peranakan Ongol Menggunakan Analisis Terminal Restriction Fragment Length Polymorphism (T-RFLP)" @default.
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