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- W127988985 abstract "Publisher Summary This chapter discusses the establishment and culture of insulin-secreting β cell lines. The most successful methods for isolating islets from large mammals, including man can provide up to 200,000 islets/ pancreas. The aim of this chapter is to describe the various types of lines available, as well as their functional characteristics. The identification of insulin-containing cells in a clone or fine cannot be made by the mere observation of living cultures because permanent cells vary in shape and size and, usually, do not contain sufficient amounts of secretory granules for a consistent detection under phase contrast. Granules are much less abundant in the β cell clones presently available than in control primary β cells and in the tumors from which permanent β cell lines have been derived. Conventional electron microscopy is also of limited value because the secretory granules of most cloned β cells vary in morphology and are often rather different from typical β granules." @default.
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- W127988985 date "1990-01-01" @default.
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- W127988985 title "[14] Establishment and culture of insulin-secreting β cell lines" @default.
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- W127988985 doi "https://doi.org/10.1016/0076-6879(90)92072-l" @default.
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