FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W128405569> ?p ?o ?g. }

• W128405569 endingPage "6" @default.
• W128405569 startingPage "3281" @default.
• W128405569 abstract "Clinical studies have demonstrated that intensive insulin therapy causes a transient worsening of retinopathy. The mechanisms underlying the initial insulin-induced deterioration of retinal status in patients with diabetes remain unknown. Vascular endothelial growth factor (VEGF) is known to be operative in the pathogenesis of diabetic retinopathy. The current study was conducted to characterize the effect of insulin on retinal VEGF gene expression in vitro and in vivo.The effect of insulin on VEGF expression in vivo was examined by in situ hybridization studies of rat retinal VEGF transcripts. To examine the mechanisms by which insulin regulates VEGF expression, human retinal pigment epithelial (RPE) cells were exposed to insulin, and VEGF mRNA levels were quantified with RNase protection assays (RPAs). Conditioned media from insulin-treated RPE cells were assayed for VEGF protein and capillary endothelial cell proliferation. The capacity of insulin to stimulate the VEGF promoter linked to a luciferase reporter gene was characterized in transient transfection assays.Insulin increased VEGF mRNA levels in the ganglion, inner nuclear, and RPE cell layers. In vitro, insulin increased VEGF mRNA levels in human RPE cells and enhanced VEGF promoter activity without affecting transcript stability. Insulin treatment also increased VEGF protein levels in conditioned RPE cell media in a dose-dependent manner with a median effective concentration of 5 nM. The insulin-conditioned RPE cell media stimulated capillary endothelial cell proliferation, an effect that was completely blocked by anti-VEGF neutralizing antibody.Insulin increases VEGF mRNA and secreted protein levels in RPE cells through enhanced transcription of the VEGF gene. Intensive insulin therapy may cause a transient worsening of retinopathy in patients with diabetes through increased retinal VEGF gene expression." @default.
• W128405569 created "2016-06-24" @default.
• W128405569 creator A5014984328 @default.
• W128405569 creator A5016285905 @default.
• W128405569 creator A5062313936 @default.
• W128405569 creator A5070793093 @default.
• W128405569 creator A5074001163 @default.
• W128405569 creator A5083155699 @default.
• W128405569 creator A5085600986 @default.
• W128405569 date "1999-12-01" @default.
• W128405569 modified "2023-10-01" @default.
• W128405569 title "Insulin-induced vascular endothelial growth factor expression in retina." @default.
• W128405569 cites W1481366355 @default.
• W128405569 cites W1497853576 @default.
• W128405569 cites W1517718326 @default.
• W128405569 cites W1582105780 @default.
• W128405569 cites W1602460865 @default.
• W128405569 cites W1974568895 @default.
• W128405569 cites W1974785275 @default.
• W128405569 cites W1976704017 @default.
• W128405569 cites W1982220314 @default.
• W128405569 cites W1987699137 @default.
• W128405569 cites W1991958278 @default.
• W128405569 cites W1997324775 @default.
• W128405569 cites W2000663727 @default.
• W128405569 cites W2001622673 @default.
• W128405569 cites W2008511815 @default.
• W128405569 cites W2011467776 @default.
• W128405569 cites W2015058903 @default.
• W128405569 cites W2018304885 @default.
• W128405569 cites W2019759067 @default.
• W128405569 cites W2028796640 @default.
• W128405569 cites W2033631750 @default.
• W128405569 cites W2035353185 @default.
• W128405569 cites W2037623358 @default.
• W128405569 cites W2044293101 @default.
• W128405569 cites W2057611314 @default.
• W128405569 cites W2062594120 @default.
• W128405569 cites W2064739543 @default.
• W128405569 cites W2075086348 @default.
• W128405569 cites W2078089300 @default.
• W128405569 cites W2094557444 @default.
• W128405569 cites W2112431699 @default.
• W128405569 cites W2128535483 @default.
• W128405569 cites W2134812217 @default.
• W128405569 cites W2137893922 @default.
• W128405569 cites W2143787351 @default.
• W128405569 cites W2145922680 @default.
• W128405569 cites W2164923772 @default.
• W128405569 cites W2171538398 @default.
• W128405569 cites W2313488710 @default.
• W128405569 cites W2322256252 @default.
• W128405569 cites W2324588265 @default.
• W128405569 cites W2325201568 @default.
• W128405569 cites W2337454357 @default.
• W128405569 cites W2434989288 @default.
• W128405569 cites W2611036568 @default.
• W128405569 cites W2769264260 @default.
• W128405569 cites W3141481798 @default.
• W128405569 cites W46679973 @default.
• W128405569 cites W67545546 @default.
• W128405569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10586954" @default.
• W128405569 hasPublicationYear "1999" @default.
• W128405569 type Work @default.
• W128405569 sameAs 128405569 @default.
• W128405569 citedByCount "39" @default.
• W128405569 countsByYear W1284055692012 @default.
• W128405569 countsByYear W1284055692013 @default.
• W128405569 countsByYear W1284055692014 @default.
• W128405569 countsByYear W1284055692015 @default.
• W128405569 countsByYear W1284055692016 @default.
• W128405569 countsByYear W1284055692017 @default.
• W128405569 countsByYear W1284055692019 @default.
• W128405569 countsByYear W1284055692020 @default.
• W128405569 crossrefType "journal-article" @default.
• W128405569 hasAuthorship W128405569A5014984328 @default.
• W128405569 hasAuthorship W128405569A5016285905 @default.
• W128405569 hasAuthorship W128405569A5062313936 @default.
• W128405569 hasAuthorship W128405569A5070793093 @default.
• W128405569 hasAuthorship W128405569A5074001163 @default.
• W128405569 hasAuthorship W128405569A5083155699 @default.
• W128405569 hasAuthorship W128405569A5085600986 @default.
• W128405569 hasConcept C126322002 @default.
• W128405569 hasConcept C134018914 @default.
• W128405569 hasConcept C146285616 @default.
• W128405569 hasConcept C167734588 @default.
• W128405569 hasConcept C169760540 @default.
• W128405569 hasConcept C170493617 @default.
• W128405569 hasConcept C2775960820 @default.
• W128405569 hasConcept C2777025900 @default.
• W128405569 hasConcept C2777093970 @default.
• W128405569 hasConcept C2779306644 @default.
• W128405569 hasConcept C2779829184 @default.
• W128405569 hasConcept C555293320 @default.
• W128405569 hasConcept C71924100 @default.
• W128405569 hasConcept C86803240 @default.
• W128405569 hasConceptScore W128405569C126322002 @default.
• W128405569 hasConceptScore W128405569C134018914 @default.

• next