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- W128519166 abstract "Abstract A modified angiotensin I radioimmunoassay method has been developed for the determination of plasma angiotensinase activity (PAA). Three nanograms of synthetic isoleucine-5-angiotensin I were incubated with 0.5 ml. of undiluted plasma at 37 °C, pH 7.4, in the presence of an inhibitor of converting enzyme (3 × 10 −3 M bipyridine). Undegraded angiotensin I was measured in triplicate 0.1 ml. aliquots of the incubation mixture by radioimmunoassay. The coefficient of variability with this method was 12.97 per cent. The maximal velocity of the PAA reaction occurred at 10 minutes of incubation. PAA was not significantly different in men and women; for the combined group of normal subjects, PAA was 4.21 ± 1.3 per cent (mean ± 1 S.D.) degradation of angiotensin 1 per minute." @default.
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- W128519166 date "1972-01-01" @default.
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- W128519166 title "Measurement of plasma angiotensinase activity by a radioimmunoassay method." @default.
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