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- W128522081 abstract "The release of lactate dehydrogenase (LDH) in the culture medium can represent a good qualitative and quantitative indicator of cellular death. An overview of the kinetics of LDH release in batch and continous cultures, with both suspension and adherent cell lines is presented. Under controlled conditions the intracellular LDH activity of living cells is relatively constant during the culture. It varies however with the pH, the dissolved oxygen level, the nature of the carbon source and the type of cells. Monitoring the release kinetics of LDH in the culture medium allows the evaluation of the actual growth and death rate of cells inside bioreactors. KEY WORDS : Lactate dehydrogenase, animal cells, cell death, enzyme release, intracellular activity, kinetics, monitoring." @default.
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- W128522081 date "1991-01-01" @default.
- W128522081 modified "2023-09-26" @default.
- W128522081 title "POTENTIAL AND PITFALLS OF USING LDH RELEASE FOR THE EVALUATION OF ANIMAL CELL DEATH KINETICS" @default.
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- W128522081 doi "https://doi.org/10.1016/b978-0-7506-1103-9.50105-0" @default.
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