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• W12858345 abstract "The feasibility of using municipal solid waste leachate as carbon source for theproduction of polyhydroxyalkanoates (PHA) was investigated in this study. Leachatesfrom MSW in Kuala Lumpur area were subjected to anaerobic treatment under specifiedcondition for the production of organic acids. Two types of leachates were used, i.e. freshleachate from the city council garbage trucks and combined leachate from several cells atthe sanitary landfill. Initially, prior to the PHA production, the characteristics (pH, COD,BODs, ammonium, total solid, suspended solid, organic acids and heavy metals) of themunicipal solid waste leachate were analysed. The effect of pH and inoculum on organicacids production during anaerobic treatment was evaluated. Treatments were carried outat 30°C for 7-10 days under different conditions of pH, i.e. uncontrolled pH, adjusted toinitial pH 7, controlled at pH 7 for 24 hours, and continuous control at pH 7 and pH 5.5.The production of organic acids from fresh leachate was highest when the initial pH wasadjusted to pH 7 with no further pH control. About 45 g/L total organic acids wasproduced, comprising of 28 g/L lactic acid, 8 g/L acetic acid and 9 g/L propionic acid.Based on the initial BODs, the organic acid yield was about 80%. In contrast, with thecombined landfill leachate the highest organic acids obtained was only 14 gIL when thepH was controlled at pH 5.5, with acetic acid as the main product. Temperature at 37°Cgave the highest production on the second day. When the fresh leachate was autoclavedand seeded with 10% fermented kitchen garbage, the highest organic acids achieved werebetween 34-37 g/L. The highest selectivity of lactic acid (85%) was achieved duringtreatment of fresh leachate seeded with kitchen garbage without any pH adjustment.Overall, the results showed that the fresh leachate could be effectively converted to 45g/L total organic acids by anaerobic treatment when the initial pH was adjusted to pH 7.The supernatant collected from the treatment of highest organic acids productionwas used in the second stage for the production of polyhydroxyalkanoate (PHA).Ralstonia eutropha was used for the production of PHA using leachate as carbon sourcefor the batch and fed-batch fermentation. In batch fermentation using shake flask, 3.4 g/LPHA was produced which gave 86% g PHAlg cell. In fed-batch fermentation themaximum PHA concentration was 6.9 g/L, corresponding to 85% (gig) of cell dryweight. The PHA produced was plastic-like and resembles low density polyethylene.Thus the fresh leachate proved to be a suitable carbon source for organic acids andsubsequently used in PHA production." @default.
• W12858345 created "2016-06-24" @default.
• W12858345 creator A5062413048 @default.
• W12858345 date "2003-01-01" @default.
• W12858345 modified "2023-09-27" @default.
• W12858345 title "Comparison of Municipal and Kitchen Waste Leachates for the Production of Organic Acids and Polyhydroxyalkanoates (PHA)" @default.
• W12858345 hasPublicationYear "2003" @default.
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