Matches in SemOpenAlex for { <https://semopenalex.org/work/W1293411> ?p ?o ?g. }
- W1293411 endingPage "84" @default.
- W1293411 startingPage "75" @default.
- W1293411 abstract "Carbon Labeling Systems (CLS) are large equation systems that describe the dynamics of labeled carbon atoms in a metabolic network. The rapid solution of these systems is the algorithmic backbone of C Metabolic Flux Analysis (MFA) which has become one of the most widely used tools in Metabolic Engineering. A new algorithm is presented for the solution of CLS which is not based on iteration schemes or numerical linear algebra methods but on path tracing of labeled particles. It is shown that the set of all paths from the system input to the internal network nodes directly gives the clue to an explicit solution of CLS. The promising potential of this new solution algorithm are outlined. 1 Metabolic Flux Analysis In recent years Metabolic Flux Analysis (MFA) by using C isotopes has become one of the most widely used tools in Metabolic Engineering [Wi01, W02]. MFA allows to determine quantitatively all fluxes in the central metabolism of a micro organism or higher cell. The metabolic flux maps resulting from this analysis serve to compare different strains of a micro organism to diagnose the effects of a genetic manipulation or even give hints to further improve the production capabilities of a given organism. MFA is based on a carbon labeling experiment where C labeled substrates are fed to the cells. The C isotopes are then distributed all over the metabolic network due to the metabolic activity. Finally, the enrichment of C isotopes in the intra-cellular metabolite pools tends to an isotopically stationary state, which means that constant fractions of unlabeled and labeled carbon atoms are encountered in all pools. In this state the isotope enrichment is measured by NMR or MS instruments [Sz98]. From this measurement data the metabolic fluxes are estimated based on a mathematical model of the carbon labeling dynamics in the system. The sole biological knowledge that is required for this procedure is the biochemical structure of the metabolic network, and the carbon atom transitions in each single reaction step. This knowledge is rather well established for central metabolism, but the method can also be used to distinguish between network variances." @default.
- W1293411 created "2016-06-24" @default.
- W1293411 creator A5018520418 @default.
- W1293411 creator A5080366910 @default.
- W1293411 creator A5091011177 @default.
- W1293411 date "2004-01-01" @default.
- W1293411 modified "2023-09-26" @default.
- W1293411 title "Kleene's theorem and the solution of metabolic carbon labeling systems" @default.
- W1293411 cites W1655990431 @default.
- W1293411 cites W1983301762 @default.
- W1293411 cites W1989845358 @default.
- W1293411 cites W1992170087 @default.
- W1293411 cites W2017721591 @default.
- W1293411 cites W2059902696 @default.
- W1293411 cites W2088884003 @default.
- W1293411 cites W2089499069 @default.
- W1293411 cites W2091963605 @default.
- W1293411 cites W2096767559 @default.
- W1293411 cites W2127359939 @default.
- W1293411 cites W2189563134 @default.
- W1293411 hasPublicationYear "2004" @default.
- W1293411 type Work @default.
- W1293411 sameAs 1293411 @default.
- W1293411 citedByCount "1" @default.
- W1293411 countsByYear W12934112014 @default.
- W1293411 crossrefType "proceedings-article" @default.
- W1293411 hasAuthorship W1293411A5018520418 @default.
- W1293411 hasAuthorship W1293411A5080366910 @default.
- W1293411 hasAuthorship W1293411A5091011177 @default.
- W1293411 hasConcept C101810790 @default.
- W1293411 hasConcept C110872660 @default.
- W1293411 hasConcept C111919701 @default.
- W1293411 hasConcept C119767625 @default.
- W1293411 hasConcept C121332964 @default.
- W1293411 hasConcept C137858568 @default.
- W1293411 hasConcept C138673069 @default.
- W1293411 hasConcept C151730666 @default.
- W1293411 hasConcept C152662350 @default.
- W1293411 hasConcept C160941953 @default.
- W1293411 hasConcept C164304813 @default.
- W1293411 hasConcept C178790620 @default.
- W1293411 hasConcept C181199279 @default.
- W1293411 hasConcept C185592680 @default.
- W1293411 hasConcept C186060115 @default.
- W1293411 hasConcept C18903297 @default.
- W1293411 hasConcept C190729725 @default.
- W1293411 hasConcept C192989942 @default.
- W1293411 hasConcept C2993678126 @default.
- W1293411 hasConcept C41008148 @default.
- W1293411 hasConcept C55493867 @default.
- W1293411 hasConcept C60644358 @default.
- W1293411 hasConcept C62231903 @default.
- W1293411 hasConcept C62520636 @default.
- W1293411 hasConcept C6350086 @default.
- W1293411 hasConcept C68709404 @default.
- W1293411 hasConcept C71924100 @default.
- W1293411 hasConcept C86803240 @default.
- W1293411 hasConceptScore W1293411C101810790 @default.
- W1293411 hasConceptScore W1293411C110872660 @default.
- W1293411 hasConceptScore W1293411C111919701 @default.
- W1293411 hasConceptScore W1293411C119767625 @default.
- W1293411 hasConceptScore W1293411C121332964 @default.
- W1293411 hasConceptScore W1293411C137858568 @default.
- W1293411 hasConceptScore W1293411C138673069 @default.
- W1293411 hasConceptScore W1293411C151730666 @default.
- W1293411 hasConceptScore W1293411C152662350 @default.
- W1293411 hasConceptScore W1293411C160941953 @default.
- W1293411 hasConceptScore W1293411C164304813 @default.
- W1293411 hasConceptScore W1293411C178790620 @default.
- W1293411 hasConceptScore W1293411C181199279 @default.
- W1293411 hasConceptScore W1293411C185592680 @default.
- W1293411 hasConceptScore W1293411C186060115 @default.
- W1293411 hasConceptScore W1293411C18903297 @default.
- W1293411 hasConceptScore W1293411C190729725 @default.
- W1293411 hasConceptScore W1293411C192989942 @default.
- W1293411 hasConceptScore W1293411C2993678126 @default.
- W1293411 hasConceptScore W1293411C41008148 @default.
- W1293411 hasConceptScore W1293411C55493867 @default.
- W1293411 hasConceptScore W1293411C60644358 @default.
- W1293411 hasConceptScore W1293411C62231903 @default.
- W1293411 hasConceptScore W1293411C62520636 @default.
- W1293411 hasConceptScore W1293411C6350086 @default.
- W1293411 hasConceptScore W1293411C68709404 @default.
- W1293411 hasConceptScore W1293411C71924100 @default.
- W1293411 hasConceptScore W1293411C86803240 @default.
- W1293411 hasLocation W12934111 @default.
- W1293411 hasOpenAccess W1293411 @default.
- W1293411 hasPrimaryLocation W12934111 @default.
- W1293411 hasRelatedWork W124488796 @default.
- W1293411 hasRelatedWork W145337578 @default.
- W1293411 hasRelatedWork W1515962586 @default.
- W1293411 hasRelatedWork W1578921938 @default.
- W1293411 hasRelatedWork W1911715606 @default.
- W1293411 hasRelatedWork W1965367951 @default.
- W1293411 hasRelatedWork W199643677 @default.
- W1293411 hasRelatedWork W2120607863 @default.
- W1293411 hasRelatedWork W2126821981 @default.
- W1293411 hasRelatedWork W2129033235 @default.