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- W131465131 abstract "This chapter describes the methods used to study the immune system of Xenopus. The classical distinction between T and B cells can be made in Xenopus where in vitro collaboration experiments have unequivocally proved the participation of carrier-specific helper T cells in the immune response of hapten-specific B cells. This collaboration, as in mammals, is controlled by major histocompatibility complex (MHC)-encoded molecules present on the lymphocyte surface. For in vivo responses, animals, larvae, or adults can be immunized with dinitrophenylated keyhole limpet hemocyanin or cellular antigens. For in vitro secondary responses, lymphocytes are prepared from primed animals and cultured with antigen at a final concentration of 1–10 μg/mL. Conditions for a primary response in vitro to this antigen have not yet been well defined. In many instances and especially when using chimeras, it is necessary to know which cells are contributed by which part of the chimera. In Xenopus, a good nuclear marker has been described between Xenopus borealis and Xenopus laevis. In some instances, when MHC compatibility is necessary, such markers cannot be used. In those cases, the best marker remains the ploidy marker." @default.
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- W131465131 date "1985-01-01" @default.
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- W131465131 title "Methods Used to Study the Immune System of Xenopus (Amphibia, Anura)" @default.
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- W131465131 doi "https://doi.org/10.1016/b978-0-12-442703-7.50031-0" @default.
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