FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W131569124> ?p ?o ?g. }

• W131569124 abstract "Mast cells (MCs) are key effector cells in various types of inflammatory conditions. The MC secretory granules contain inflammatory mediators such as histamine, heparin proteoglycan (PG), cytokines and various heparin-binding proteases, including tryptases, chymases and carboxypeptidase A. Previously, a mouse strain with a defect in its heparin biosynthesis was produced by targeting the gene for NDST-2 (N-deacetylase/N-sulfotransferase-2). These mice showed reduced levels of MC inflammatory mediators such as histamine and various heparin-binding proteases, including chymases, tryptases, and carboxypeptidase A. By using this mouse strain, we found that chymase in complex with heparin PG degraded fibronectin, suggesting a role for chymase in the regulation of connective tissue composition. Further, we found that chymase/heparin PG complexes degraded and thereby inactivated both thrombin and plasmin, suggesting an additional role for chymase in regulation of extravascular coagulation and fibrinolysis. However, although our findings implicated chymase in these processes, it was not possible to exclude the contribution to the observed activities by other MC components that are influenced by the knockout of NDST-2. Out of the different mouse chymases, mouse MC protease 4 (mMCP-4) has the most similar tissue distribution, heparin-binding and angiotensin I-converting properties as the only identified human chymase. Thus, mMCP-4 may be the closest homologue to human chymase and we therefore chose to target the gene for mMCP-4. A mouse strain with a targeted inactivation of the mMCP-4 gene was generated. This mouse strain displayed defects in the regulation of thrombin and in fibronectin turnover, demonstrating a key role for mMCP-4 in these processes. To address the role of MC proteases in the activation of matrix metalloproteases (MMPs) we used both the NDST-2 and mMCP-4 deficient mice. An analysis of peritoneal cells and tissue extracts from these mice revealed the presence of both pro-MMP-9 and active enzyme (MMP-9) in WT mice, but only the proform of MMP-9 was found in knockout mice. We also found that mMCP-4 can regulate the activation of pro-MMP-2 in the same manner. Our findings suggest that mMCP-4 plays a critical role in the activation of both pro-MMP-2 and pro-MMP-9 in vivo. The mMCP-4 knockout led to the accumulation of the fibronectin and collagen, resulting in fibrotic signs in the skin of the mMCP-4-/- mice. Possibly, mMCP-4-mediated activation of pro-MMP-2 and -9 may provide a link between MCs and processes that are regulated by MMPs." @default.
• W131569124 created "2016-06-24" @default.
• W131569124 creator A5072315135 @default.
• W131569124 date "2004-01-01" @default.
• W131569124 modified "2023-09-27" @default.
• W131569124 title "Biological function of mast cell chymase" @default.
• W131569124 cites W100157191 @default.
• W131569124 cites W101168984 @default.
• W131569124 cites W1472929708 @default.
• W131569124 cites W1481220364 @default.
• W131569124 cites W1483486599 @default.
• W131569124 cites W1483520967 @default.
• W131569124 cites W1487496227 @default.
• W131569124 cites W1494869776 @default.
• W131569124 cites W1511593691 @default.
• W131569124 cites W1519269060 @default.
• W131569124 cites W1530655559 @default.
• W131569124 cites W1531766605 @default.
• W131569124 cites W1547330548 @default.
• W131569124 cites W1549183351 @default.
• W131569124 cites W1570863417 @default.
• W131569124 cites W1571278720 @default.
• W131569124 cites W1575824270 @default.
• W131569124 cites W1577884079 @default.
• W131569124 cites W1582826730 @default.
• W131569124 cites W1586061801 @default.
• W131569124 cites W1593238714 @default.
• W131569124 cites W1594492118 @default.
• W131569124 cites W1596543212 @default.
• W131569124 cites W1599496006 @default.
• W131569124 cites W1603416910 @default.
• W131569124 cites W1625181872 @default.
• W131569124 cites W1630458667 @default.
• W131569124 cites W1638151543 @default.
• W131569124 cites W1644900513 @default.
• W131569124 cites W1668468287 @default.
• W131569124 cites W166946010 @default.
• W131569124 cites W167584193 @default.
• W131569124 cites W1792289918 @default.
• W131569124 cites W1886491539 @default.
• W131569124 cites W1947012228 @default.
• W131569124 cites W1959914147 @default.
• W131569124 cites W1963612704 @default.
• W131569124 cites W1968665107 @default.
• W131569124 cites W1968773217 @default.
• W131569124 cites W1968894752 @default.
• W131569124 cites W1969525788 @default.
• W131569124 cites W1970490335 @default.
• W131569124 cites W1971540677 @default.
• W131569124 cites W1973366653 @default.
• W131569124 cites W1973602059 @default.
• W131569124 cites W1974468764 @default.
• W131569124 cites W1975068237 @default.
• W131569124 cites W1975996690 @default.
• W131569124 cites W1976229113 @default.
• W131569124 cites W1976962628 @default.
• W131569124 cites W1977986376 @default.
• W131569124 cites W1978399820 @default.
• W131569124 cites W1980832959 @default.
• W131569124 cites W1981818027 @default.
• W131569124 cites W1982091128 @default.
• W131569124 cites W1982330819 @default.
• W131569124 cites W1982643537 @default.
• W131569124 cites W1983337695 @default.
• W131569124 cites W1983746522 @default.
• W131569124 cites W1984295704 @default.
• W131569124 cites W1985469117 @default.
• W131569124 cites W1986732560 @default.
• W131569124 cites W1989254741 @default.
• W131569124 cites W1989849372 @default.
• W131569124 cites W1990383981 @default.
• W131569124 cites W1990459952 @default.
• W131569124 cites W1993585253 @default.
• W131569124 cites W1994028989 @default.
• W131569124 cites W1998312296 @default.
• W131569124 cites W1998533172 @default.
• W131569124 cites W1998783767 @default.
• W131569124 cites W1998950078 @default.
• W131569124 cites W2000431416 @default.
• W131569124 cites W2001696702 @default.
• W131569124 cites W2006862534 @default.
• W131569124 cites W2010715204 @default.
• W131569124 cites W2011345555 @default.
• W131569124 cites W2013582082 @default.
• W131569124 cites W2014671375 @default.
• W131569124 cites W2016574653 @default.
• W131569124 cites W2017220914 @default.
• W131569124 cites W2017431991 @default.
• W131569124 cites W2018210258 @default.
• W131569124 cites W2020096963 @default.
• W131569124 cites W2021019784 @default.
• W131569124 cites W2023606932 @default.
• W131569124 cites W2023926028 @default.
• W131569124 cites W2026960965 @default.
• W131569124 cites W2029389633 @default.
• W131569124 cites W2029708509 @default.
• W131569124 cites W2032807973 @default.
• W131569124 cites W2033330205 @default.
• W131569124 cites W2034203371 @default.
• W131569124 cites W2034386687 @default.

• next