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- W132713759 abstract "This chapter presents monosaccharide and oligosaccharide analysis of recombinant eiythropoietin electro-transferred onto polyvinylidene fluoride membranes. In a study described in the chapter, glass-distilled deionized water was used for the preparation of all buffers, eluents, and electrophoresis solutions. The storage flask and spigot were glass throughout to prevent contact with tubings that might support microbial growth and yield artifactual sugars during monosaccharide analysis. SDS-PAGE (12.5%) was performed using mini-gels run in a Tall Mighty Small unit at constant amperage (20 mA) using an LKB Multidrive 3.5 kV power supply. The kinetics of monosaccharide release from soluble rEPO and rEPO electro-transferred onto PVDF was compared. The time-dependent release of neutral, amino, and the 6-deoxy sugars (Fuc) from rEPO in solution (1A) was similar to that observed for electro-transferred rEPO (1B). The percent recovery of monosaccharides upon comparing electrotransferred and soluble rEPO hydrolysates was Gal, 80%; GlcN, 91%; Man, 70%; Fuc, 83%; and GalN, 89%." @default.
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- W132713759 date "1993-01-01" @default.
- W132713759 modified "2023-10-18" @default.
- W132713759 title "Monosaccharide and Oligosaccharide Analysis of Recombinant Erythropoietin Electro-transferred onto Polyvinylidene Fluoride Membranes" @default.
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- W132713759 doi "https://doi.org/10.1016/b978-0-12-058757-5.50020-2" @default.
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