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- W133279088 abstract "RNA interference (RNAi) is a powerful gene-knockdown technology that has been applied for functional genetic loss-of-function studies in many model eukaryotic systems, including embryonic stem cells (ESCs). Application of RNAi in ESCs allows for dissection of mechanisms by which ESCs self-renew and maintain pluripotency, and also specifying particular cell types needed for cell-replacement therapies. Potent RNAi response can be induced by expression of an microRNA-embedded short-hairpin RNA (shRNA(mir)) cassette that is integrated in the genome by virus infection or site-specific recombination at a defined locus. In this chapter, I will provide detailed protocols to perform shRNA(mir)-mediated RNAi studies in mouse ESCs using retrovirus infection and loxP site-directed recombination for efficient constitutive and inducible gene knockdown, respectively." @default.
- W133279088 created "2016-06-24" @default.
- W133279088 creator A5008841048 @default.
- W133279088 date "2017-01-01" @default.
- W133279088 modified "2023-10-16" @default.
- W133279088 title "Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs" @default.
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- W133279088 doi "https://doi.org/10.1007/978-1-4939-7108-4_17" @default.
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