Matches in SemOpenAlex for { <https://semopenalex.org/work/W133348346> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W133348346 endingPage "40" @default.
- W133348346 startingPage "32" @default.
- W133348346 abstract "Human corneal endothelial cells are thought to have limited capacity for proliferation. Little is known about the mechanisms that regulate the proliferation of these cells. The authors introduced oncogenes into human corneal endothelial cells to modulate proliferation. In addition, they sought to establish cell lines to facilitate study of human corneal endothelial cells.Early-passage human corneal endothelial cells were transduced with disabled retrovirus (pLXSN16E6/E7) coding for the human papilloma virus type 16 transforming oncoproteins E6 and E7. Early-passage cells were also stably transfected by electroporation with the pMTV-D305 plasmid vector, in which SV40 large T antigen (SV40 LTAg) mRNA expression is positively regulated by the mouse mammary tumor virus promoter. Expression of E6/E7 mRNA or SV40 LTAg mRNA in cell lines was monitored with the polymerase chain reaction. SV40 LTAg protein expression was detected by immunocytology and Western blot analysis.Human corneal endothelial cells were efficiently infected with disabled retrovirus coding for E6/E7, and seven strains of cells have continued active proliferation for more than 50 population doublings (PD) (< 8 control PD). E6/E7 mRNA was expressed by each cell strain. E6/E7 transformed cells proliferate rapidly and form a monolayer of cells with a high degree of contact inhibition. Transfection with pMTV-D305 is less efficient, and only a single strain was developed. pMTV-D305-transfected endothelial cells (dexamethasone induced) proliferated at a lower rate than E6/E7-transduced cells or cells transfected with a vector (pSV3neo) in which SV40 LTAg is constitutively regulated. In the absence of dexamethasone, the proliferation of pMTV-D305-transfected cells was even slower, but cells continued to produce SV40 LTAg mRNA and protein. The latter results indicated that SV40 LTAg mRNA continued to be synthesized at significant levels in pMTV-D305-transfected cells in the absence of the inducer dexamethasone.This study suggests that human corneal endothelial cells have a high capacity for proliferation. Thus, cell division is normally controlled in human corneal endothelial cells by poorly characterized, but efficient, mechanisms. Because the E6 and E7 proteins, as well as the SV40 large T antigen, specifically bind to and interfere with the activity of the retinoblastoma (RB) and p53 tumor suppressor proteins, our results suggest that these proteins have critical roles in regulating the proliferation of human corneal endothelial cells." @default.
- W133348346 created "2016-06-24" @default.
- W133348346 creator A5000918396 @default.
- W133348346 creator A5004249007 @default.
- W133348346 creator A5070413526 @default.
- W133348346 creator A5076538321 @default.
- W133348346 creator A5086544825 @default.
- W133348346 date "1995-01-01" @default.
- W133348346 modified "2023-10-15" @default.
- W133348346 title "Expression of E6/E7 or SV40 large T antigen-coding oncogenes in human corneal endothelial cells indicates regulated high-proliferative capacity." @default.
- W133348346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7822156" @default.
- W133348346 hasPublicationYear "1995" @default.
- W133348346 type Work @default.
- W133348346 sameAs 133348346 @default.
- W133348346 citedByCount "51" @default.
- W133348346 countsByYear W1333483462012 @default.
- W133348346 countsByYear W1333483462013 @default.
- W133348346 countsByYear W1333483462015 @default.
- W133348346 countsByYear W1333483462016 @default.
- W133348346 countsByYear W1333483462022 @default.
- W133348346 crossrefType "journal-article" @default.
- W133348346 hasAuthorship W133348346A5000918396 @default.
- W133348346 hasAuthorship W133348346A5004249007 @default.
- W133348346 hasAuthorship W133348346A5070413526 @default.
- W133348346 hasAuthorship W133348346A5076538321 @default.
- W133348346 hasAuthorship W133348346A5086544825 @default.
- W133348346 hasConcept C123012128 @default.
- W133348346 hasConcept C134018914 @default.
- W133348346 hasConcept C1491633281 @default.
- W133348346 hasConcept C153911025 @default.
- W133348346 hasConcept C159047783 @default.
- W133348346 hasConcept C202751555 @default.
- W133348346 hasConcept C2522874641 @default.
- W133348346 hasConcept C2775901793 @default.
- W133348346 hasConcept C2776992346 @default.
- W133348346 hasConcept C2778431562 @default.
- W133348346 hasConcept C54009773 @default.
- W133348346 hasConcept C54355233 @default.
- W133348346 hasConcept C55493867 @default.
- W133348346 hasConcept C81885089 @default.
- W133348346 hasConcept C86803240 @default.
- W133348346 hasConceptScore W133348346C123012128 @default.
- W133348346 hasConceptScore W133348346C134018914 @default.
- W133348346 hasConceptScore W133348346C1491633281 @default.
- W133348346 hasConceptScore W133348346C153911025 @default.
- W133348346 hasConceptScore W133348346C159047783 @default.
- W133348346 hasConceptScore W133348346C202751555 @default.
- W133348346 hasConceptScore W133348346C2522874641 @default.
- W133348346 hasConceptScore W133348346C2775901793 @default.
- W133348346 hasConceptScore W133348346C2776992346 @default.
- W133348346 hasConceptScore W133348346C2778431562 @default.
- W133348346 hasConceptScore W133348346C54009773 @default.
- W133348346 hasConceptScore W133348346C54355233 @default.
- W133348346 hasConceptScore W133348346C55493867 @default.
- W133348346 hasConceptScore W133348346C81885089 @default.
- W133348346 hasConceptScore W133348346C86803240 @default.
- W133348346 hasIssue "1" @default.
- W133348346 hasLocation W1333483461 @default.
- W133348346 hasOpenAccess W133348346 @default.
- W133348346 hasPrimaryLocation W1333483461 @default.
- W133348346 hasRelatedWork W1991797776 @default.
- W133348346 hasRelatedWork W2080740418 @default.
- W133348346 hasRelatedWork W2374858880 @default.
- W133348346 hasRelatedWork W2376734542 @default.
- W133348346 hasRelatedWork W2384261171 @default.
- W133348346 hasRelatedWork W2388714568 @default.
- W133348346 hasRelatedWork W2389012941 @default.
- W133348346 hasRelatedWork W2391802113 @default.
- W133348346 hasRelatedWork W29331271 @default.
- W133348346 hasRelatedWork W3142015144 @default.
- W133348346 hasVolume "36" @default.
- W133348346 isParatext "false" @default.
- W133348346 isRetracted "false" @default.
- W133348346 magId "133348346" @default.
- W133348346 workType "article" @default.